Abbreviation
Recombinant Human MICB protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human MICB at 2 μg/mL can bind Anti-MICA/B recombinant antibody(CSB-RA56059MA1HU). The EC50 is 0.9180-1.062 ng/mL.
Species
Homo sapiens (Human)
Expression Region
23-309aa
Target Protein Sequence
AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAEDVLGAETWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYNGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRTD
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human MICB at 2 μg/ml can bind Anti-MICA/B recombinant antibody(CSB-RA56059MA1HU). The EC50 is 0.9180-1.062 ng/mL.
Description
MICB functions as a stress-induced ligand for the activating receptor NKG2D on natural killer cells and CD8+ T cells, making it a critical node in immune surveillance and tumor immunology. This mammalian-expressed protein spanning residues 23–309 captures the complete extracellular domain architecture required for physiologically relevant NKG2D engagement, and its binding to an anti-MICA/B recombinant antibody yields an EC50 of 0.9180–1.062 ng/mL in functional ELISA, demonstrating the conformational integrity necessary for receptor-ligand interaction studies and therapeutic antibody epitope mapping. The quantified binding kinetics support its use in competitive inhibition assays to screen blocking antibodies, in SPR or BLI experiments for affinity characterization of NKG2D-targeting biologics, and as a positive control in immune checkpoint research focused on NK cell activation pathways. Purity exceeding 95% by SDS-PAGE and endotoxin below 1.0 EU/μg align with standards expected in cell-based functional assays where low contaminant levels are essential.
