Abbreviation
Recombinant Human MICA protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human MICA at 2 μg/mL can bind Anti-MICA/B recombinant antibody (CSB-RA56059MA1HU). The EC50 is 0.9163-1.108 ng/mL.
Alternative Names
MHC Class I chain-related protein; MHC class I polypeptide-related sequence A; MICA
Species
Homo sapiens (Human)
Expression Region
24-309aa
Target Protein Sequence
EPHSLRYNLTVLSWDGSVQSGFLAEVHLDGQPFLRYDRQKCRAKPQGQWAEDVLGNKTWDRETRDLTGNGKDLRMTLAHIKDQKEGLHSLQEIRVCEIHEDNSTRSSQHFYYDGELFLSQNVETEEWTVPQSSRAQTLAMNVRNFLKEDAMKTKTHYHAMHADCLQELRRYLESSVVLRRTVPPMVNVTRSEASEGNITVTCRASSFYPRNITLTWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICQGEEQRFTCYMEHSGNHSTHPVPSGKVLVLQSHWQT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human MICA at 2 μg/ml can bind Anti-MICA/B recombinant antibody (CSB-RA56059MA1HU). The EC50 is 0.9163-1.108 ng/mL.
Description
MICA functions as a stress-induced ligand for the activating NKG2D receptor on NK cells and CD8+ T cells, making it a critical node in tumor immunosurveillance and autoimmune pathology. This mammalian-expressed construct spanning residues 24–309 captures the complete extracellular domain, preserving the native glycosylation and disulfide architecture required for physiological NKG2D engagement. Functional ELISA validation demonstrates robust antibody binding with an EC50 of 0.9163–1.108 ng/mL, confirming that the folded protein retains conformational epitopes and supports applications including therapeutic antibody epitope mapping, competitive inhibition screening for NKG2D-blocking biologics, and SPR-based affinity characterization of MICA-targeting drug candidates. The combination of >95% purity and <1.0 EU/μg endotoxin meets the quality thresholds commonly required for ligand-receptor interaction assays and immune checkpoint research where low endotoxin is essential to avoid confounding NK cell activation.
