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Recombinant Human Lysine-specific demethylase 2A (KDM2A), partial

Recombinant Human Lysine-specific demethylase 2A (KDM2A), partial

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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

KDM2A

Uniprot NO.

Q9Y2K7

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

1-1162

Target Protein Sequence

MEPEEERIRY SQRLRGTMRR RYEDDGISDD EIEGKRTFDL EEKLHTNKYN ANFVTFMEGK DFNVEYIQRG GLRDPLIFKN SDGLGIKMPD PDFTVNDVKM CVGSRRMVDV MDVNTQKGIE MTMAQWTRYY ETPEEEREKL YNVISLEFSH TRLENMVQRP STVDFIDWVD NMWPRHLKES QTESTNAILE MQYPKVQKYC LMSVRGCYTD FHVDFGGTSV WYHIHQGGKV FWLIPPTAHN LELYENWLLS GKQGDIFLGD RVSDCQRIEL KQGYTFVIPS GWIHAVYTPT DTLVFGGNFL HSFNIPMQLK IYNIEDRTRV PNKFRYPFYY EMCWYVLERY VYCITNRSHL TKEFQKESLS MDLELNGLES GNGDEEAVDR EPRRLSSRRS VLTSPVANGV NLDYDGLGKT CRSLPSLKKT LAGDSSSDCS RGSHNGQVWD PQCAPRKDRQ VHLTHFELEG LRCLVDKLES LPLHKKCVPT GIEDEDALIA DVKILLEELA NSDPKLALTG VPIVQWPKRD KLKFPTRPKV RVPTIPITKP HTMKPAPRLT PVRPAAASPI VSGARRRRVR CRKCKACVQG ECGVCHYCRD MKKFGGPGRM KQSCVLRQCL APRLPHSVTC SLCGEVDQNE ETQDFEKKLM ECCICNEIVH PGCLQMDGEG LLNEELPNCW ECPKCYQEDS SEKAQKRKME ESDEEAVQAK VLRPLRSCDE PLTPPPHSPT SMLQLIHDPV SPRGMVTRSS PGAGPSDHHS ASRDERFKRR QLLRLQATER TMVREKENNP SGKKELSEVE KAKIRGSYLT VTLQRPTKEL HGTSIVPKLQ AITASSANLR HSPRVLVQHC PARTPQRGDE EGLGGEEEEE EEEEEEDDSA EEGGAARLNG RGSWAQDGDE SWMQREVWMS VFRYLSRREL CECMRVCKTW YKWCCDKRLW TKIDLSRCKA IVPQALSGII KRQPVSLDLS WTNISKKQLT WLVNRLPGLK DLLLAGCSWS AVSALSTSSC PLLRTLDLRW AVGIKDPQIR DLLTPPADKP GQDNRSKLRN MTDFRLAGLD ITDATLRLII RHMPLLSRLD LSHCSHLTDQ SSNLLTAVGS STRYSLTELN MAGCNKLTDQ TLIYLRRIAN VTLIDLRGCK QITRKACEHF ISDLSINSLY CLSDEKLIQK IS

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-ARNTL/BMAL1 heterodimer and RORA in a catalytically-independent manner.

Gene References into Functions

KDM2A-SF forms distinct nuclear heterochromatic bodies in an HP1a dependent manner PMID:28816576
Authors conclude that KDM2A functions as an oncogene in breast cancer by upregulating JAG1 to promote stemness, chemoresistance and angiogenesis. PMID:27029061
Identify a nucleosome interaction module within KDM2A consisting of a CXXC type zinc finger, a PHD domain and a newly identified Heterochromatin Protein 1 (HP1) interaction motif that mediates direct binding between KDM2A and HP1. This nucleosome interaction module enables KDM2A to decode nucleosomal H3K9me3 modification in addition to CpG methylation signals. PMID:28180290
The miR-29b/KDM2A axis was involved in the RUNX3-mediated inhibition of gastric cancer cell proliferation and metastasis. PMID:27497248
KDM2A-WWTR1 fusion is associated with ossifying fibromyxoid tumors. PMID:27537276
Silencing KDM2A, a histone demethylase and BCL6 co-repressor, de-repressed SFRP2 transcription by increasing histone H3K4 and H3K36 methylation at the SFRP2 promoter. PMID:27074224
Findings suggest that amplification and overexpression of the KDM2A short isoform is critical in breast cancer progression. PMID:26207617
In this study, KDM2A was identified as a novel substrate of ATM. DSB enhanced the interaction between ATM and KDM2A, which induced ATM-mediated phosphorylation of KDM2A at T632. PMID:25823024
The results suggest that under mild glucose starvation AMP-activated kinase induces KDM2A-dependent reduction of rRNA transcription to control cell proliferation. PMID:26416883
FBXL11 is proposed as a novel component of the circadian clock that regulates the circadian gene expression by a so far unknown mechanism. PMID:26037310
The protein lysine demethylases Kdm2a and Kdm2b regulate the turnover of non-phosphorylated beta-catenin specifically within the nucleus via direct interaction with the fourth and fifth armadillo repeats. PMID:26004508
Upregulation of KDM2A is very important in the progression of gastric cancer. PMID:25245333
a regulatory role for KDM2A in breast cancer cell invasion and migration, through the regulation of E2F1 function PMID:25029110
KDM2A binds to the rDNA promoter with unmethylated CpG sequences via the CxxC-ZF domain PMID:24553073
KDM2A represses histone deacetylase 3 and has a role in tumorigenicity of lung cancer cells PMID:24482232
KDMA2 is frequently overexpressed in NSCLC tumors & cell lines. It is needed for in vitro proliferation & invasion. KDM2A activated ERK1/2 by epigenetic repression of DUSP3 expression via demethylation of dimethylated H3K36 at the DUSP3 locus. PMID:24200691
KDM2A is a H3K4 demethylase that regulates cell proliferation through p15(INK4B) and p27(Kip1) in stem cells from apical papilla. PMID:23559091
FBXL11 inhibited osteo/dentinogenic differentiation potential in MSC cells by associating with BCOR, then increasing histone K4/36 methylation in Epiregulin promoter to repress Epiregulin transcription. PMID:23074094
results not only show that differently spliced transcripts from a gene result in totally opposite outcomes, but also present critical evidence of the complicated activities of KDM2A, which contains all of the five domains PMID:22635273
The oncometabolite 2-hydroxyglutarate is a competitive inhibitor of multiple alpha-ketoglutarate-dependent dioxygenases, including histone demethylases, prolyl hydroxylases, and the TET family of 5-methlycytosine hydroxylases. PMID:21251613
KDM2A represses the transcription of ribosomal RNA. KDM2A was localized in nucleoli and bound to the ribosomal RNA gene promoter. PMID:20379134
CpG islands recruit KDM2A protein, a histone H3 lysine 36 demethylase. PMID:20417597
Data describe a NF-kappaB regulatory pathway that is driven by reversible lysine methylation of p65, carried out by nuclear receptor-binding SET domain-containing protein 1 (NSD1) and F-box and leucine-rich repeat protein 11 (FBXL11). PMID:20080798
KDM2A is required to sustain centromeric integrity and genomic stability, particularly during mitosis. PMID:19001877
Data show that high levels of FBXL11 block the ability of NFkappaB to bind to DNA or activate gene expression. PMID:19805303

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Subcellular Location

Nucleus, nucleoplasm. Note=Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.

Protein Families

JHDM1 histone demethylase family

Tissue Specificity

Widely expressed, with highest levels in brain, testis and ovary, followed by lung.

Database Links

HGNC: 13606

UNIGENE: Hs.124147

KEGG: hsa:22992

STRING: 9606.ENSP00000432786

OMIM: 605657

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