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Recombinant Human L-dopachrome tautomerase (DCT),Partial

Recombinant Human L-dopachrome tautomerase (DCT),Partial

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Code: CSB-EP006562HU

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Size:

20ug100ug1mg(1mg*1 or 500ug*2)

US$224

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Express system: E.coli

Species: Homo sapiens (Human)

Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

DCT

Uniprot NO.

P40126

Research Area

others

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

24-472aa

Target Protein Sequence

QFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNALEGFDKADGTLDSQVMSLHNLVHSFLNGTNALPHSAANDPIFVVLHSFTDAIFDEWMKRFNPPADAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTSDQLGYSYAIDLPVSVEETPGWPTT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

71.3 kDa

Protein Length

Topological Domain

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

Tris-based buffer,50% glycerol

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Troubleshooting and FAQs

Protein FAQs

Datasheet & COA

Please contact us to get it.

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Description

E.coli-expressed L-dopachrome tautomerase (DCT) CSB-EP006562HU is a recombinant fusion protein of partial DCT coupled to a 10xHis-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. This protein consists of the topological domain (23-108aa) of human DCT and has a calculated molecular weight of 71.3 kDa. The SDS-PAGE showed an about 80 kDa molecular mass of this protein and determined the purity of over 90%. In-stock DCT proteins are offered now. In addition to producing specific antibodies, this recombinant DCT protein may be used in the studies of melanogenesis.

L-DCT or TRP-2 is an important regulatory enzyme involved in melanin synthesis. It also executes proliferative, immunogenic, and anti-apoptotic roles in melanoma cells. L-DCT/TRP-2 is also a marker for amelanotic and melanotic melanoma. Studies showed that A3B5 (N-(3,5-dimethylphenyl)-3-methoxybenzamide) effectively inhibited melanin production by the degradation of TRP-2.

Customer Reviews and Q&A

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■ Q&A

I was hoping you can supply more information about the expression of this protein in E.coli. Was it expressed as a soluble construct or from inclusion bodies with refolding? I am trying to express my own protein however I cannot get soluble protein and my protein does not work as well as the protein I bought from you. Are you able to help with this information? CSB-EP006562HU Recombinant Human L-dopachrome tautomerase (DCT)

Very nice to receive your inquiry. This protein is expressed from inclusion bodies with refolding.
You could also try to do inclusion bodies, then purify, and finally refold. The final buffer could refer to our datasheet.

Target Background

Function(From Uniprot)

Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).

Gene References into Functions

High DCT expression is associated with progressive multiple sclerosis. PMID:28923927
High DCT expression is associated with HPV16 Infection. PMID:28095444
Data indicate that the caveolin-1 (CAV1) control on dopachrome tautomerase (DCT) gene expression, DCT post-translational processing, and subcellular distribution is cell phenotype-dependent. PMID:27053106
The effect of DCT on UVR DNA damage responses and survival pathways in melanocytes was examined by knockdown experiments using melanoma cells, neonatal melanoblasts in monoculture and in co-culture with keratinocytes. PMID:25346513
Report dopachrome tautomerase expression in mealanocytic tumors. PMID:24709887
The results suggested that the miRNAs may be involved in MITF regulation of TYR, TYRP1 and TYRP2, which provides a new clue for understanding the role of miRNAs in melanocyte dysfunctional disease. PMID:22898827
Data suggest that macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT) act cooperatively to inhibit steady-state phosphorylation and activation of AMPK in LKB1 wild type and LKB1 mutant non-small cell lung carcinomas cells. PMID:22988252
human TRP2 counteracts apoptotic cell death induction, possibly by means of its L-dopachrome tautomerase activity, and negatively affects the p53 pathway PMID:20520707
Trp2 SNP2 allele is a risk factor for the development and severity of degenerative disc disease in a Chinese Han population. PMID:20356546
Data suggest that OTX2 may regulate retinal pigment epithelium (RPE)-specific target genes, such as DOPAchrome tautomerase (DCT), thereby maintaining the homeostasis of RPE. PMID:12559959
Because TRP-2(181-190) overlaps with the known HLA-A*0201-presented epitope TRP-2(180-188), an 11mer peptide encompassing both epitopes might be of specific value for vaccination of a broad population of melanoma patients. PMID:15856458
Transgenic Dct regulates neural progenitor cell proliferation in a mouse model. PMID:16857183
No genetic susceptibility or increased risk attributed to the tyrosinase gene family in Vogt-Koyanagi-Harada disease in Japanese. PMID:17200659
Anemonin, an active compound of C. crassifolia, inhibits melanin synthesis by inhibiting the transcription of the genes encoding TYR, TRP1, and TRP2. PMID:17766092
we studied a possible role of dopachrome tautomerase in the oxidative stress response in the amelanotic WM35 melanoma cell line. PMID:18206123
We observe strong evidence for positive selection for DCT in Asians PMID:18312627
Tyrosinase-, gp100-, or TRP-2-specific CD8(+) T cells could not be identified in the peripheral blood of individuals with vitiligo [TRP-2]. PMID:18337837
TRP-2 acts on quinone metabolites other than DOPAchrome, e.g., in the catecholamine pathway, and limits their deleterious effects. PMID:18674612
tyrosinase-related protein 2 (TRP-2) transcript is not detected in the peripheral blood mononuclear cells (PBMC) of vitiligo patients PMID:19284502
Expression profiling demonstrated that the DCT-expressing cell population expressed adrenergic and muscarinic receptors and displayed transcriptional profiles distinct from dermal melanocytes PMID:19855129