Abbreviation
Recombinant Human IL1R2 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human IL1B(CSB-MP011614HU) at 5 μg/mL can bind Human IL1R2. The EC50 is 89.65-100.5 ng/mL.
Alternative Names
Interleukin-1 receptor type 2; IL-1R-2; IL-1RT-2; IL-1RT2; CD121 antigen-like family member B; CDw121b; IL-1 type II receptor; Interleukin-1 receptor beta (IL-1R-beta); Interleukin-1 receptor type II; Interleukin-1 receptor type 2, membrane form (mIL-1R2; mIL-1RII); Interleukin-1 receptor type 2, soluble form (sIL-1R2; sIL-1RII); CD121b; IL1R2; IL1RB
Species
Homo sapiens (Human)
Expression Region
14-343aa
Target Protein Sequence
FTLQPAAHTGAARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSPRINLTWHKNDSARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTRNASYCDKMSIELRVFENTDAFLPFISYPQILTLSTSGVLVCPDLSEFTRDKTDVKIQWYKDSLLLDKDNEKFLSVRGTTHLLVHDVALEDAGYYRCVLTFAHEGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSRLTIPCKVFLGTGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENYIEVPLIFDPVTREDLHMDFKCVVHNTLSFQTLRTTVKE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal hFc1-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human IL1B(CSB-MP011614HU) at 5 μg/ml can bind Human IL1R2. The EC50 is 89.65-100.5 ng/mL.
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The purity of IL1R2 was greater than 90% as determined by SEC-HPLC
Description
IL-1 receptor type 2 functions as a decoy receptor that sequesters IL-1β without triggering downstream signaling, making it a critical negative regulator in inflammatory cascades and a target for therapeutic modulation in autoimmune and inflammatory diseases. This mammalian-expressed construct spanning residues 14–343 captures the complete extracellular ligand-binding domain, ensuring native glycosylation patterns that preserve conformational epitopes essential for accurate receptor-ligand interaction studies. Functional ELISA validation demonstrates specific binding to immobilized IL-1β with an EC50 of 89.65–100.5 ng/mL, providing quantitative evidence that this protein supports ligand-binding assays, competitive inhibition screens for IL-1 pathway modulators, and blocking antibody characterization in drug discovery programs targeting inflammatory signaling. The combination of >95% purity by SDS-PAGE, >90% purity by SEC-HPLC, and endotoxin levels below 1.0 EU/μg satisfies the quality criteria typical for surface plasmon resonance affinity measurements, bio-layer interferometry kinetic studies, and therapeutic antibody epitope mapping workflows.
