Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
ATOD2; Epidermal filaggrin; FILA_HUMAN; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
Species
Homo sapiens (Human)
Expression Region
3838-4061aa
Target Protein Sequence
DSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
Recombinant Human Filaggrin/FLG is a partial-length protein expressed with an N-terminal 6xHis-tag in the yeast. Its expression region corresponds to 3838-4061aa of human FLG protein. Its purity was determined by SDS-PAGE and reached up to 90%. This recombinant FLG protein may be used to synthesize antibodies against FLG or on the studies of FLG-associated signal transduction. FLG proteins in-stock are available.
FLG is an epidermal differentiation protein that plays a fundamental role in the development and maintenance of the skin barrier. The binding of FLG with the intermediate keratin filaments leads to their aggregation and compaction, making cells collapse and flattening that is required for squame biogenesis. Within the squames, FLG undergoes further proteolysis and degrade into hygroscopic amino acids and other derivatives, which make up the natural moisturizing factor (NMF) responsible for water retention in the stratum corneum. FLG mutations perturb the skin barrier function, which causes ichthyosis vulgaris, increase the risk of atopic dermatitis and other atopic diseases.
