Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Canstatin; CO4A2_HUMAN; COL4A 2; Col4a2; Collagen alpha 2(IV) chain; Collagen; type IV; alpha 2
Species
Homo sapiens (Human)
Expression Region
1493-1712aa
Target Protein Sequence
VKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
The recombinant Human COL4A2 was expressed with the amino acid range of 1493-1712. This COL4A2 protein is theoretically predicted to have a molecular weight of 28.3 kDa. This COL4A2 protein is produced using e.coli expression system. The COL4A2 gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant COL4A2 protein during the following stages.
The human collagen alpha-2 (IV) chain, encoded by the COL4A2 gene, is a vital component of type IV collagen, a major structural protein found in basement membranes. COL4A2 is essential for maintaining the structural integrity of tissues and organs, especially in the vascular system and basement membranes of various organs. Mutations in COL4A2 can lead to Alport syndrome, a hereditary disorder affecting the kidneys, eyes, and ears. Research on COL4A2 encompasses understanding its role in basement membrane function, tissue development, and its association with various pathological conditions. Investigating COL4A2 provides insights into extracellular matrix biology and has implications for potential therapeutic strategies targeting collagen-related disorders.
