Abbreviation
Recombinant Human CHI3L1 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Human CHI3L1 at 2 μg/mL can bind Anti-CHI3L1 recombinant antibody (CSB-RA005346MA2HU). The EC50 is 15.06-22.43 ng/mL.
②CHI3L1 Recombinant Monoclonal Antibody (CSB-RA005346MA2HU) captured on Protein A Chip can bind Recombinant Human CHI3L1 with an affinity constant of 2.52 nM as detected by MetaSPR Assay (WeSPRTM 200).
Alternative Names
Chitinase-3-like protein 1; 39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40
Species
Homo sapiens (Human)
Expression Region
22-383aa
Target Protein Sequence
YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CHI3L1 at 2 μg/ml can bind Anti-CHI3L1 recombinant antibody (CSB-RA005346MA2HU). The EC50 is 15.06-22.43 ng/mL.
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Activity
CHI3L1 Recombinant Monoclonal Antibody (CSB-RA005346MA2HU) captured on Protein A Chip can bind Recombinant Human CHI3L1 with an affinity constant of 2.52 nM as detected by MetaSPR Assay (WeSPRTM 200).
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The purity of CHI3L1 was greater than 95% as determined by SEC-HPLC
Description
CHI3L1, a secreted glycoprotein implicated in inflammation and tumor progression, presents unique challenges for recombinant production due to its complex glycosylation requirements and propensity for aggregation. This full-length mature protein (aa 22–383) expressed in mammalian cells demonstrates robust binding activity with an EC50 of 15.06–22.43 ng/mL in functional ELISA and a KD of 2.52 nM by surface plasmon resonance, confirming proper folding and epitope accessibility. These quantitative binding parameters support use in antibody screening campaigns, where consistent antigen presentation is critical for identifying high-affinity candidates, as well as in competitive binding assays designed to map epitopes or assess antibody cross-reactivity. The protein meets the purity and endotoxin criteria typical for immunoassay development (>95% purity, <1.0 EU/μg), providing a suitable basis for generating standard curves in diagnostic ELISA formats or serving as a capture antigen in multiplex immunoassays targeting inflammatory biomarkers.
