Abbreviation
Recombinant Human CEACAM1 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human CEACAM1 at 2 μg/mL can bind Anti-CEACAM1 recombinant antibody (CSB-RA005157MA1HU). The EC50 is 0.3677-0.4528 ng/mL.
Alternative Names
Cell adhesion molecule CEACAM1; Biliary glycoprotein 1; Carcinoembryonic antigen-related cell adhesion molecule 1; CD66a; CEACAM1; BGP
Species
Homo sapiens (Human)
Expression Region
35-428aa
Target Protein Sequence
QLTTESMPFNVAEGKEVLLLVHNLPQQLFGYSWYKGERVDGNRQIVGYAIGTQQATPGPANSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPETQDTTYLWWINNQSLPVSPRLQLSNGNRTLTLLSVTRNDTGPYECEIQNPVSANRSDPVTLNVTYGPDTPTISPSDTYYRPGANLSLSCYAASNPPAQYSWLINGTFQQSTQELFIPNITVNNSGSYTCHANNSVTGCNRTTVKTIIVTELSPVVAKPQIKASKTTVTGDKDSVNLTCSTNDTGISIRWFFKNQSLPSSERMKLSQGNTTLSINPVKREDAGTYWCEVFNPISKNQSDPIMLNVNYNALPQENGLSPG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human CEACAM1 at 2 μg/ml can bind Anti-CEACAM1 recombinant antibody (CSB-RA005157MA1HU). The EC50 is 0.3677-0.4528 ng/mL.
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The purity of CEACAM1 was greater than 90% as determined by SEC-HPLC
Description
CEACAM1 mediates homophilic and heterophilic cell–cell interactions critical to immune regulation and tumor progression, making quantitative assessment of its binding properties essential for cancer biology studies. This yeast-expressed construct spanning residues 35–428 demonstrates specific antibody recognition in functional ELISA with an EC50 of 0.37–0.45 ng/mL, confirming that the extracellular domain retains epitope accessibility suitable for antibody development and validation workflows. The low endotoxin level (< 1.0 EU/μg) combined with > 95% purity supports use in cell-based assays where inflammatory artifacts must be minimized, including cell adhesion and spreading experiments that probe CEACAM1-mediated intercellular contacts. Researchers investigating tumor metastasis mechanisms or immune cell trafficking can apply this protein to dissect CEACAM1's role in cell migration and invasion, leveraging the validated binding activity to establish dose-response relationships in ligand–receptor interaction studies.
