Abbreviation
Recombinant Human CLEC9A protein, partial, Biotinylated (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Anti-CLEC9A recombinant antibody (CSB-RA740922MA1HU) at 2 μg/mL can bind Human CLEC9A protein. The EC50 is 2.915-3.520 ng/mL.
Alternative Names
C-type lectin domain family 9 member A; CD370; CLEC9A; UNQ9341/PRO34046
Species
Homo sapiens (Human)
Expression Region
57-241aa
Target Protein Sequence
KLLQVSTIAMQQQEKLIQQERALLNFTEWKRSCALQMKYCQAFMQNSLSSAHNSSPCPNNWIQNRESCYYVSEIWSIWHTSQENCLKEGSTLLQIESKEEMDFITGSLRKIKGSYDYWVGLSQDGHSGRWLWQDGSSPSPGLLPAERSQSANQVCGYVKSNSLLSSNCSTWKYFICEKYALRSSV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal hFc1-Avi-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Anti-CLEC9A recombinant antibody (CSB-RA740922MA1HU) at 2 μg/ml can bind Human CLEC9A protein. The EC50 is 2.915-3.520 ng/mL.
Description
CLEC9A, a C-type lectin receptor expressed on cross-presenting dendritic cells, recognizes exposed actin filaments from necrotic cells and directs antigen uptake for CD8+ T cell priming—a pathway central to anti-tumor immunity and vaccine design. This biotinylated construct spanning residues 57–241 captures the extracellular ligand-binding domain and demonstrates quantified binding activity in functional ELISA, with an EC50 of 2.915–3.520 ng/mL against an anti-CLEC9A antibody, providing a validated basis for ligand-binding assays, competitive inhibition screening, and therapeutic antibody epitope mapping. Mammalian expression supports native glycosylation and disulfide bond formation critical for C-type lectin fold integrity, while the N-terminal hFc1-Avi tag enables oriented immobilization in SPR and BLI platforms for affinity characterization and blocking antibody validation. Purity exceeding 90% by SDS-PAGE and endotoxin below 1.0 EU/μg align with standards expected in cell-based functional assays and antibody screening workflows targeting dendritic cell receptors.
