Recombinant Human Beta-enolase (ENO3), partial

In Stock
Code: CSB-RP131674h
Size:
20ug
20ug100ug1mg(1mg*1 or 500ug*2)
US$224
Quantity:
Express system: E.coli
Species: Homo sapiens (Human)
Tag Info: N-terminal 6xHis-tagged
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
ENO3
Uniprot NO.
Research Area
Metabolism
Alternative Names
2-phospho-D-glycerate hydro-lyase;Enolase 3Muscle-specific enolase ;MSESkeletal muscle enolase
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
7-432aa
Target Protein Sequence
FAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
50.1kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Troubleshooting and FAQs
Datasheet & COA
Please contact us to get it.
Images
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description

Dive into the complex world of metabolic research with our high-quality Recombinant Human ENO3 protein. Beta-enolase, also known as 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, or Skeletal muscle enolase, plays a vital role in glycolysis and glucose metabolism. This protein is essential for energy production in skeletal muscles and serves as an important biomarker in various pathological conditions.

Our Recombinant Human ENO3 protein, expressed in E. coli, features a partial construct that covers the 7-432aa region, ensuring the availability of crucial domains for your metabolic research. The N-terminal 6xHis-tag facilitates efficient protein purification and identification, simplifying your experimentation process. With a purity greater than 90% as determined by SDS-PAGE, our Recombinant Human ENO3 protein is supplied in a lyophilized powder format, guaranteeing you a high-quality product for your research needs.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
Appears to have a function in striated muscle development and regeneration.
Gene References into Functions
  1. Molecular genetic analysis of ENO3 gene revealed two novel homozygous missense mutations, (p.Asn151Ser and p.Glu187Lys)in patients presenting with recurrent rhabdomyolysis. PMID:25267339
  2. Pyridoxamine and carnosine protected enolase against the total loss of catalytic activity. PMID:21347838

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Subcellular Location
Cytoplasm.
Protein Families
Enolase family
Tissue Specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Database Links

HGNC: 3354

UNIGENE: Hs.224171

KEGG: hsa:2027

STRING: 9606.ENSP00000324105

OMIM: 131370

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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