Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Neuroscience
Alternative Names
Adrenalin oxidase; Amine oxidase (flavin containing); Amine oxidase [flavin-containing] B; AOFB_HUMAN; MAO, brain; MAO, platelet; MAO-B; MAOB; MGC26382; Monoamine oxidase B; Monoamine oxidase type B; OTTHUMP00000023166; Tyramine oxidase
Species
Homo sapiens (Human)
Expression Region
2-489aa
Target Protein Sequence
SNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIPSDAPWKAPLAEEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFVGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPMMRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDTKPEGNYAAIMGFILAHKARKLARLTKEERLKKLCELYAKVLGSLEALEPVHYEEKNWCEEQYSGGCYTTYFPPGILTQYGRVLRQPVDRIYFAGTETATHWSGYMEGAVEAGERAAREILHAMGKIPEDEIWQSEPESVDVPAQPITTTFLERHLPSV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Cytoplasmic Domain
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP013399HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MAOB.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP013399HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MAOB.
Description
Amino acids 2-489 form the expressed segment for recombinant Human MAOB. This MAOB protein is theoretically predicted to have a molecular weight of 59.3 kDa. This protein is generated in a e.coli-based system. The MAOB gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant MAOB protein during the following stages.
Human amine oxidase [flavin-containing] B (MAOB) plays a crucial role in the catabolism of biogenic amines, including neurotransmitters like dopamine, serotonin, and norepinephrine. Located in the outer mitochondrial membrane, MAOB catalyzes the oxidative deamination of these amines, contributing to the regulation of neurotransmitter levels. Its involvement in the breakdown of dopamine makes MAOB a target in neurological disorders such as Parkinson's disease. Beyond its role in neurobiology, MAOB is involved in cardiovascular health and age-related conditions. The exploration of MAOB extends across various research areas, including neuroscience, cardiology, and gerontology, emphasizing its significance in understanding and potentially treating diverse medical conditions.
