Recombinant Histone H3.3

Code
MSDS	MSDS
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Uniprot NO.

P15512

Species

Tetrahymena pyriformis

Source

Yeast

Expression Region

2-136

Target Protein Sequence

ARTKQTARK STGVKAPRKQ LATKAARKSA PVSGGVKKPH KFRPGTVALR EIRKYQKTTD LLIRKLPFQR LVRDIAMEMK NDIRFQSQAI LALQEAAEAY LVGLFEDTNL CAIHARRVTI MTKDMQLARR IRGERF

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

Macronuclear replacement variant which replaces conventional H3 in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Functions redundantly to H3.4. H3.3 deposition into chromatin is mainly transcription-associated and DNA replication-independent, but it can also enter a replication-coupled pathway. Although not essential for vegetative growth, minor H3 variants are required for producing viable conjugation progeny by affecting late developmental stages of conjugation.

Subcellular Location

Nucleus. Chromosome.

Protein Families

Histone H3 family

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