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Recombinant Haemophilus influenzae Catabolite gene activator (crp)

Recombinant Haemophilus influenzae Catabolite gene activator (crp)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

crp

Uniprot NO.

P29281

Alternative Names

crp; cap; HI_0957; cAMP-activated global transcriptional regulator CRP; Catabolite activator protein; CAP; cAMP receptor protein; CRP; cAMP regulatory protein

Species

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Source

Yeast

Expression Region

1-224

Target Protein Sequence

MSNELTEIDE VVTSSQEEAT QRDPVLDWFL THCHLHKYPA KSTLIHAGED ATTLYYVIKG SVMVSSKDDE GKEMILTYLG AGQFFGEAGL FDEGSKRSAW VKTKTTCEIA EISYKKYRQL IQANPEILMF LTAQLARRLQ NTSRQVTNLA FLDVAGRIAQ TLMNLAKQPE AMTHPDGMQI KITRQEIGQM VGCSRETVGR IIKMLEDQNL IHAHGKTIVV YGAR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

A global transcription regulator required for bacterial competence. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to regulate transcription at 54 promoters. There are 2 consensus subclasses; CRP-N (above) and CRP-S (T6 changed to C and A17 changed to G) which obligatorily also requires Sxy for expression. CRP-S sites bind DNA but do not activate transcription in the absence of Sxy. Probably induces a severe bend in DNA. Probably acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.

Database Links

KEGG: hin:HI0957

STRING: 71421.HI0957

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