Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 2 (EBNA2), partial

The expression region of this recombinant Epstein-Barr virus EBNA2 covers amino acids 247-454. The expected molecular weight for the EBNA2 protein is calculated to be 25.9 kDa. This EBNA2 recombinant protein is manufactured in yeast. The EBNA2 gene fragment has been modified by fusing the N-terminal 6xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant EBNA2 protein during the following stages.

Epstein-Barr virus Epstein-Barr nuclear antigen 2 (EBNA2) is a transcriptional activator that plays a crucial role in initiating and maintaining the viral latent infection. It regulates the expression of both viral and cellular genes, promoting the survival and proliferation of infected B cells. EBNA2 interacts with host cell transcription factors, including the cellular DNA-binding protein RBP-Jκ, leading to the activation of viral latent genes, thus contributing to the establishment of latent infection. EBNA2 is essential for the transformation of primary B cells into immortalized lymphoblastoid cell lines (LCLs). This process is a hallmark of EBV-associated B-cell malignancies. EBNA2 can modulate various cellular signaling pathways, including the Notch signaling pathway, to create a favorable environment for viral persistence and cell survival. EBNA2's role in cellular transformation is linked to the development of EBV-associated cancers, such as Burkitt's lymphoma, Hodgkin's lymphoma, and nasopharyngeal carcinoma.