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Recombinant Drosophila melanogaster Maternal protein pumilio (pum), partial

Recombinant Drosophila melanogaster Maternal protein pumilio (pum), partial

Baculovirus
Mammalian cell

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

pum

Uniprot NO.

P25822

Alternative Names

pum; CG9755; Maternal protein pumilio

Species

Drosophila melanogaster (Fruit fly)

Source

Yeast

Expression Region

1-1533

Target Protein Sequence

MKFLGGNDDR NGRGGVGVGT DAIVGSRGGV SQDAADAAGA AAAAAVGYVF QQRPSPGGVG VGVGGVGGGV PGVGAVGSTL HEAAAAEYAA HFAQKQQQTR WACGDDGHGI DNPDKWKYNP PMNPANAAPG GPPGNGSNGG PGAIGTIGMG SGLGGGGGGG AGGGNNGGSG TNGGLHHQSM AAAAANMAAM QQAAALAKHN HMISQAAAAV AAQQQHQHPH QQHPQQQQQQ QQAQNQGHPH HLMGGGNGLG NGNGLGIQHP GQQQQQQQQQ QQQQHPGQYN ANLLNHAAAL GHMSSYAQSG GSMYDHHGGA MHPGMNGGMP KQQPLGPPGA GGPQDYVYMG GQTTVPMGAA MMPPQNQYMN SSAVAAANRN AAITTSTAKK LWEKSDGKGV SSSTPGGPLH PLQIPGIGDP SSVWKDHTWS TQGENILVPP PSRAYAHGGA SDTSNSGNAG ILSPRDSTCA KVVEYVFSGS PTNKDSSLSG LEPHLRNLKF DDNDKSRDDK EKANSPFDTN GLKKDDQVTN SNGVVNGIDD DKGFNRTPGS RQPSPAEESQ PRPPNLLFPP LPFNHMLMDH GQGMGGGLGG VVGSGNGVGG GSGGGGAGGA YAAHQQMAAQ MSQLQPPMMN GVGGGMPMAA QSPMLNHQAA GPNHMESPGN LLQQQNFDVQ QLFRSQNPGL AAVATNAAAA AAAAAAATSA ASAAAAVGAP PVPNGSLQQS QQQQQQQQQQ QQQQQMHMAA ASQQFLAAQQ QAQNAAYAAQ QATSYVINPG QEAAPYMGMI AAAQMPYYGV APWGMYPGNL IPQQGTQPRR PLTPSQQGAE NQPYQVIPAF LDHTGSLLMG GPRTGTPMRL VSPAPVLVPP GATRAGPPPP QGPQLYQPQP QTAQQNLYSQ QNGSSVGGLA LNTSSLTGRR DSFDRSTSAF SPSTMDYTSS GVAAAANAVN STVAQAAAAA AAAAAARGKW PGAMSGAASG AYGALGAGNA SASPLGAPIT PPPSAQSCLL GSRAPGAESR QRQQQQQQLA AVGLPATAAA AQAAVAAAAN NMFGSNSSIF SNPLAIPGTA AVAAAAAAAA AANSRQVAAT AAAAAAVAAA AGGVGGAPQP GRSRLLEDFR NQRYPNLQLR DLANHIVEFS QDQHGSRFIQ QKLERATAAE KQMVFSEILA AAYSLMTDVF GNYVIQKFFE FGTPEQKNTL GMQVKGHVLQ LALQMYGCRV IQKALESISP EQQQEIVHEL DGHVLKCVKD QNGNHVVQKC IECVDPVALQ FIINAFKGQV YSLSTHPYGC RVIQRILEHC TAEQTTPILD ELHEHTEQLI QDQYGNYVIQ HVLEHGKQED KSILINSVRG KVLVLSQHKF ASNVVEKCVT HATRGERTGL IDEVCTFNDN ALHVMMKDQY ANYVVQKMID VSEPTQLKKL MTKIRPHMAA LRKYTYGKHI NAKLEKYYMK ITNPITVGTG AGGVPAASSA AAVSSGATSA SVTACTSGSS TTTTSTTNSL ASPTICSVQE NGSAMVVEPS SPDASESSSS VVSGAVNSSL GPIGPPTNGN VVL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of deadenylase complexes leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Mediates post-transcriptional silencing of E2f mRNA by binding to its 3'-UTR and promoting miRNA regulation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole.

Gene References into Functions

The specificity of a complex between Drosophila RNA-binding protein Pumilio and its target RNA of Nanos has been described. PMID:27729296
Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity. PMID:27482653
reductions in the expression of the Pumilio translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. the PUM complex is a target of LSD1 regulation. PMID:26438601
Pumilio has a novel mRNA decay function during neural development. Decreased expression stabilized predicted neural mRNA targets. A Pumilio recognition element is needed to trigger reporter-transcript decay in the nervous system. PMID:25896902
Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches PMID:22391052
Pumilio complexes repress dE2F1 levels PMID:22345517
Data show that the extended 3' UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3' lengthening phenomenon. PMID:21896737
The studies suggest that, in cystoblasts, Bam and Bgcn may directly inhibit Pum/Nos activity to promote differentiation of germ line stem cells. PMID:20018853
affects anterior determination in drosophila embryo; temporally regulates bicoid (bcd) mRNA translation via evolutionarily conserved Nanos response elements (NRE) in its 3'UTR PMID:12015297
a translaitonal repressor that regulates neuronal excitability PMID:12136020
Pumilio represses translation of differentiation-promoting mRNAs in germline stem cells. PMID:15668176
A characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO was identified. PMID:16537387
Regulatory interplay between Nanos and Pum could cause a small change in Pum activity to be amplified into a large shift in the balance between GluRIIA and GluRIIB synapses. PMID:19403823