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Recombinant Drosophila melanogaster Dorsal-related immunity factor Dif (Dif), partial

Recombinant Drosophila melanogaster Dorsal-related immunity factor Dif (Dif), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Dif

Uniprot NO.

P98149

Alternative Names

Dif; CG6794Dorsal-related immunity factor Dif

Species

Drosophila melanogaster (Fruit fly)

Source

Yeast

Expression Region

1-667

Target Protein Sequence

MFEEAFGDIQ EIINASMELN GGATGGGSVA GAVGGGGAAH HILSQSTSLP VMPSHIPLHL QNQNMNQNLP EPSARSGPHL RIVEEPTSNI IRFRYKCEGR TAGSIPGMNS SSETGKTFPT IEVCNYDGPV IIVVSCVTSD EPFRQHPHWL VSKEEADACK SGIYQKKLPP EERRLVLQKV GIQCAKKLEM RDSLVERERR NIDPFNAKFD HKDQIDKINR YELRLCYQAF ITVGNSKVPL DPIVSSPIYG KSSELTITRL CSCAATANGG DEIIMLCEKI AKDDIEVRFY ETDKDGRETW FANAEFQPTD VFKQMAIAFK TPRYRNTEIT QSVNVELKLV RPSDGATSAP LPFEYYPNPE LLTKHNRRVA QKTVESLKRS LMSTNLHPSK QVKTSSQYTI FSKPQIATTT PQTQVSPGMP LMFPGGSPNF VQDIKMENGF MDVDSQSSQC PSVERNFASP RSNCSTVDSI PPMQMGQNQT HLYLPDATNF TFNGNFASPS SNCSTVDSIP PFQIGQRNNH MYLPENSNFP VNGCSPTHFS GGSMTPINNN NNVLINNNNN DFLSQKMSAI SIPPQGNFGI KQVYQQTQQF LPQLQPESIP YLAQSHPEQS QYQQQQQPQE QQPPADEPTQ SFSDLISSSI GMAPIDTSEL IQDIEAELNS LGIQPFK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Mediates an immune response in larvae. DIF binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of the immunity gene CECA1. Involved in the melanization immune response to bacterial challenge, possibly by acting in a Toll signaling pathway that downregulates Spn27A in response to infection. Part of a signaling pathway involving NF-kappa-B (rel) and Toll-related receptors, that functions in the apoptosis of unfit cells during cell competition. May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system.

Gene References into Functions

These results, not only revealed a novel function and modulation pattern of miR-958, but also provided a new insight into the underlying molecular mechanisms of Toll signaling in regulation of innate immunity. PMID:27974298
Fasting increases survival to cold in dif mutant Drosophila melanogaster. PMID:25663303
DIF factor seems to be involved in the response to fungal infection after a mild cold stress and in the resistance to heat. DIF seems to have no role in the increased longevity of non-infected flies and resistance to a severe cold shock. PMID:22791143
Data show that the DIF and Relish complex is detectable in whole animal extracts, suggesting that this heterodimer may function in vivo to increase the spectrum and level of antimicrobial peptide production in response to different infections. PMID:20679214
Various fungi stimulated antimicrobial peptides through at least two different pathways requiring Relish and/or Dif. PMID:14985331
Dif and Dorsal control crucial aspects of the cellular immune response PMID:17060622
Molecular modeling was used to compare the tertiary structure of Dif with two other Drosophila proteins. PMID:17785540