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Recombinant Drosophila melanogaster DNA topoisomerase 2 (Top2), partial

Recombinant Drosophila melanogaster DNA topoisomerase 2 (Top2), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Top2

Uniprot NO.

P15348

Species

Drosophila melanogaster (Fruit fly)

Source

Yeast

Expression Region

1-1447

Target Protein Sequence

MENGNKALSI EQMYQKKSQL EHILLRPDSY IGSVEFTKEL MWVYDNSQNR MVQKEISFVP GLYKIFDEIL VNAADNKQRD KSMNTIKIDI DPERNMVSVW NNGQGIPVTM HKEQKMYVPT MIFGHLLTSS NYNDDEKKVT GGRNGYGAKL CNIFSTSFTV ETATREYKKS FKQTWGNNMG KASDVQIKDF NGTDYTRITF SPDLAKFKMD RLDEDIVALM SRRAYDVAAS SKGVSVFLNG NKLGVRNFKD YIDLHIKNTD DDSGPPIKIV HEVANERWEV ACCPSDRGFQ QVSFVNSIAT YKGGRHVDHV VDNLIKQLLE VLKKKNKGGI NIKPFQVRNH LWVFVNCLIE NPTFDSQTKE NMTLQQKGFG SKCTLSEKFI NNMSKSGIVE SVLAWAKFKA QNDIAKTGGR KSSKIKGIPK LEDANEAGGK NSIKCTLILT EGDSAKSLAV SGLGVIGRDL YGVFPLRGKL LNVREANFKQ LSENAEINNL CKIIGLQYKK KYLTEDDLKT LRYGKVMIMT DQDQDGSHIK GLLINFIHTN WPELLRLPFL EEFITPIVKA TKKNEELSFY SLPEFEEWKN DTANHHTYNI KYYKGLGTST SKEAKEYFQD MDRHRILFKY DGSVDDESIV MAFSKKHIES RKVWLTNHMD EVKRRKELGL PERYLYTKGT KSITYADFIN LELVLFSNAD NERSIPSLVD GLKPGQRKVM FTCFKRNDKR EVKVAQLSGS VAEMSAYHHG EVSLQMTIVN LAQNFVGANN INLLEPRGQF GTRLSGGKDC ASARYIFTIM SPLTRLIYHP LDDPLLDYQV DDGQKIEPLW YLPIIPMVLV NGAEGIGTGW STKISNYNPR EIMKNLRKMI NGQEPSVMHP WYKNFLGRME YVSDGRYIQT GNIQILSGNR LEISELPVGV WTQNYKENVL EPLSNGTEKV KGIISEYREY HTDTTVRFVI SFAPGEFERI HAEEGGFYRV FKLTTTLSTN QMHAFDQNNC LRRFPTAIDI LKEYYKLRRE YYARRRDFLV GQLTAQADRL SDQARFILEK CEKKLVVENK QRKAMCDELL KRGYRPDPVK EWQRRIKMED AEQADEEDEE EEEAAPSVSS KAKKEKEVDP EKAFKKLTDV KKFDYLLGMS MWMLTEEKKN ELLKQRDTKL SELESLRKKT PEMLWLDDLD ALESKLNEVE EKERAEEQGI NLKTAKALKG QKSASAKGRK VKSMGGGAGA GDVFPDPDGE PVEFKITEEI IKKMAAAAKV AQAAKEPKKP KEPKEPKVKK EPKGKQIKAE PDASGDEVDE FDAMVEGGSK TSPKAKKAVV KKEPGEKKPR QKKENGGGLK QSKIDFSKAK AKKSDDDVEE VTPRAERPGR RQASKKIDYS SLFSDEEEDG GNVGSDDDGN ASDDDSPKRP AKRGREDESS GGAKKKAPPK KRRAVIESDD DDIEIDEDDD DDSDFNC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. During meiosis, it disrupts heterochromatic connections between achiasmate and chiasmate homologs after spindle assembly so that chromosomes can separate at prometaphase I. During mitosis, it functions in the separation of sister chromatids by establishing amphitelic kinetochore attachments in mitotic spindles. May have a role in chromatin condensation and chromosome structure. May be involved in X-chromosome dosage compensation, perhaps by modifying the topological state of compensated genes. Regulates activity of the gypsy chromatin insulator complex by binding to mod(mdg4) and preventing its degradation.

Gene References into Functions

The Top2-Mus101 interaction is important for maintaining the fidelity of chromosome segregation during mitosis. PMID:27129233
our results suggest that the previously observed discrepancies in the chromosomal phenotypes elicited by Topo II downregulation in vertebrates might depend on slight differences in Topo II concentration and/or activity PMID:25340516
These data suggest both that Topoisomerase II is involved in the resolution of heterochromatic DNA entanglements during meiosis I and that these entanglements must be resolved in order to complete meiosis. PMID:25340780
Topo II is required to prevent Mod(mdg4)2.2 degradation and, consequently, to stabilize Su(Hw) insulator-mediated chromatin organization PMID:21304601
Topo II does have some role in mitotic chromatin condensation PMID:14600258