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Recombinant Dog Sodium/calcium exchanger 1 (SLC8A1), partial

Recombinant Dog Sodium/calcium exchanger 1 (SLC8A1), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SLC8A1

Uniprot NO.

P23685

Alternative Names

SLC8A1; NCX1; Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1; Solute carrier family 8 member 1

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Source

Yeast

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline. Required for normal embryonic heart development and the onset of heart contractions.

Gene References into Functions

knockdown of NCX1 in Madin-Darby canine kidney cells alters epithelial morphology and characteristics by destabilization of E-cadherin and induction of beta-catenin signaling. PMID:28550085
NCX1 palmitoylation is governed by a distal secondary structure element rather than by local primary sequence. PMID:28432123
Palmitoylation of NCX1 occurs in the Golgi and anchors the NCX1 large regulatory intracellular loop to membranes. PMID:26174834
These data confirm the role of NCX1 activity in regulating renal epithelial cell migration. PMID:25770213
f-loop residues 562-679 may contain the regulatory site for endogenous exchanger inhibitor peptide XIP, but this site is distinct from the Ca2+-regulatory domains of the NCX. PMID:15550690
NCX1 is a novel regulatory target for calcineurin; depressed NCX activity might contribute to the etiology of in vivo cardiac hypertrophy and dysfunction occurring under conditions in which both calcineurin and protein kinase C are chronically activated PMID:15557343
Endothelin-1 enhances the activity of Na+/Ca2+ exchanger type 1 in renal epithelial cells. PMID:15838289
Results suggest that Ca2+ overload via NCX1 plays a critical role in hypoxia/reoxygenation-induced renal tubular injury, and that endothelin-1 aggravates the cell damage through the activation of NCX1. PMID:15838349
crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster, indicative of a highly sensitive Ca2+ sensor PMID:16774926
analysis of transmembrane segments 1, 2, and 6 in canine NCX1 PMID:16785232
Insertion of fluorescent-tagged Na+-Ca+ exchanger proteins within the large intracellular loop of canine NCX1 is well tolerated and leads to active transporters. PMID:17158867
Ca(2+) elicits a local transition to a well-ordered coordination geometry in the CBD1-binding site of NCX1. PMID:18280495
These results indicate that NCX-1 can be a biomarker for chronic valvular disease in dogs and is a potential biomarker for severity of heart disease. PMID:19000248
Kinetic and equilibrium properties of regulatory calcium sensors of NCX1 protein. PMID:19141619
Phospholemman regulates cardiac Na+/Ca2+ exchanger by interacting with the exchanger's proximal linker domain. PMID:19158404
Structural and mutational analyses indicate that only two of the four Ca(2+) ions that bind to CBD1 of NCX1.1 are important for regulating exchanger activity. PMID:19332552
Data suggest that the electrostatic switch in CBD1 and the associated conformational change are necessary for Na+/Ca2+ exchanger activation. PMID:19667209
role of the CBD1-CBD2 linker in slow dissociation of Ca2+ from the regulatory two-domain tandem of NCX1 PMID:20587421
The expression of NCX-1 in dogs with chronic mitral valve insufficiency or azotemia showed that the latter does not affect NCX-1 expression. PMID:21054542
Data show that the Ca(2+)-dependent movements detected between paired NCXs were abolished by mutating the Ca(2+) coordination sites in CBD1. PMID:21209335
the second Ca(2+)-binding domain of NCX1 binds Mg2+ with high affinity PMID:21928827
mu-Calpain-mediated deregulation of cardiac, brain, and kidney NCX1 splice variants. PMID:22209698
The Ca(2+)-driven interdomain switch described here represents a general mechanism for initial conduction of regulatory signals in NCX variants. PMID:22768191
Population shift underlies Ca2+-induced regulatory transitions in the sodium-calcium exchanger (NCX). PMID:23798674