Recombinant Dog Insulin (INS), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

INS

Uniprot NO.

P01321

Species

Canis familiaris (Dog) (Canis lupus familiaris)

Source

Yeast

Expression Region

25-54

Target Protein Sequence

FVNQHL CGSHLVEALY LVCGERGFFY TPKA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

■ Q&A

A few questions :
1. Is this the beta-chain? Seems so when comparing the amino acid sequence.
2. Could you estimate if the tag removal can be successful?
3. If it is not the full length insulin, is it possible to get the full length insulin?
I am intend to do mass-spec for these proteins. Is there any recommendation that which expression system will be best for this application?

Very nice to receive your inquiry.
1) Yes. This is Insulin B chain.
2) We could try to remove the tag. The previous success rate of our tag removal service is 75%-86%.
Now we could simultaneously connect multiple carriers for tag removal and the success rate will be higher than previous. If we succeed in removing the tag, we will charge for extra cost.
The lead time for tag removal service will be 3 working days.
3) If the customer just need conventional mass spectrometry identification, we could perform MS for all the four expression systems.

Target Background

Function(From Uniprot)

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Gene References into Functions

The etiology of blindness in dogs with pituitary dependent hypercortisolism, including the role of adiponectin, interleukin 6, insulin, and nitric oxide, is reported. PMID:22381588
Gluconeogenic flux can be rapidly inhibited by high insulin levels in canines. PMID:19755527

Hide All

Subcellular Location

Secreted.

Protein Families

Insulin family

Database Links

KEGG: cfa:483665

STRING: 9615.ENSCAFP00000040273

Promotions