Recombinant Dog Endothelin-1 (EDN1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

EDN1

Uniprot NO.

P13206

Alternative Names

EDN1Endothelin-1; ET-1; Preproendothelin-1; PPET1) [Cleaved into: Big endothelin-1]

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Source

Yeast

Expression Region

53-73

Target Protein Sequence

CSCSSLMD KECVYFCHLD IIW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Cytoplasmic domain

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.

Gene References into Functions

These findings suggest that elevated serum big ET-1 could be used as a diagnostic marker for canine hemangiosarcoma . PMID:25920760
This study showed that canine osteosarcoma cells express endothelin-1 and functional endothelin A receptors; increases in bone alkaline phosphatase activity are associated with osteoblastic OS lesions. PMID:26426813
There was a progressive increase in ET-1 and ETAR expression from benign tumour to grade 1 and grade 2 malignant mammary tumours with a decrease of expression in grade 3 carcinomas. Co-localization of ET-1 and ETAR was observed in benign mammary tumours. PMID:25816929
These findings point to the potential of serum big ET-1 as a clinical marker for cardiopulmonary and neoplastic diseases in dogs. PMID:24447631
Renal denervation substantially decreased BP and also significantly decreased the plasma levels of renin-angiotensin system and ET-1. PMID:24496516
These results showed that endothelin-1 and endothelin receptor A are overexpressed in canine ovarian tumours, suggesting a potential role of these two molecules in canine ovarian carcinogenesis. PMID:20210880
ET-1 production in macrophage cell line DH82 was increased in response to stimulation with intact and viable gram-positive and gram-negative bacteria. PMID:20207425
The results showed that there was an interaction between ET-1 and NO in a VILI (ventilator-induced lung injury )model and changing the balance of ET-1 and NO levels might contribute to the pathophysiologic process of VILI. PMID:20497708
Report endothelin levels in experimental diabetes combined with cardiac hypertrophy. PMID:15838277
Report changes in endothelin-1 gene expression during myocardial ischemia and reperfusion. PMID:15838278
Intrapericardial adenine nucleosides induce elevation of endothelin-1 concentration in the pericardial space of the dog heart. PMID:15838286
The results confirm that intrapericardial catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. PMID:15838288
Report changes in endothelin-1/big endothelin-1 levels and action potential duration during myocardial ischemia-reperfusion in dogs with and without ventricular fibrillation. PMID:15838324
although ET(A) receptor-signaling is sensitized by induction of the metabolic syndrome, endothelin-mediated coronary vasoconstriction does not significantly contribute to coronary dysfunction at this early stage of prediabetes PMID:16627363
These results may potentially explain resting perfusion abnormalities or heterogeneity by clinical PET that may persist or only partially improve after adenosine stress perfusion imaging in the absence of myocardial scar and flow-limiting stenosis. PMID:16951040
Alterations in blood levels of endothelin-1, atrial natriuretic peptide, aldosterone, and norepinephrine in dogs with and without dilated cardiomyopathy are reported. PMID:17338155
Early pulmonary hypertension in heart failure is characterized by altered vasoreactivity and increased ET-1 and VEGF2 signalling. PMID:17993509
peripheral NO and ET-1 release modulates the cardiac contractility through myocardial ET-A receptor activation and mast cell degranulation. PMID:18788466
The use of Plasma atrial natriuretic peptide (proANP 31-67), B-type natriuretic peptide (Nt-proBNP) and endothelin-1 (ET-1) concentrations as disease markers in dogs with valvular heart disease is reported. PMID:19585255