Recombinant Dog Dystrophin (DMD), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

DMD

Uniprot NO.

O97592

Alternative Names

DMDDystrophin

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Source

Yeast

Expression Region

1-3680

Target Protein Sequence

MLWWEEVEDC YEREDVQKKT FTKWVNAQFS KFGKQHIENL FSDLQDGRRL LDLLEGLTGQ KLPKEKGSTR VHALNNVNKA LRVLQKNNVD LVNIGSTDIV DGNHKLTLGL IWNIILHWQV KNVMKNIMAG LQQTNSEKIL LSWVRQSTRN YPQVNVINFT TSWSDGLALN ALIHSHRPDL FDWNSVVCQQ SATQRLEHAF NIAKYQLGIE KLLDPEDVAT TYPDKKSILM YITSLFQVLP QQVSIEAIQE VEMLPRPSQV TREEHFQIHH QMHYSQQITV SLAQGYERAP SFPKPRFKSY AYTQAAYVTT SDPTRSPLPS QHLETPEDKS FGRSLTETEA NLDSYQTALE EVLSWLLSAE DALQAQGEIS NDVEEVKEQF HTHEGYMMDL TSHQGRVGNV LQLGSQLIGT GKLSEDEETE VQEQMNLLNS RWECLRVASM EKQSNLHKVL MDLQNQQLKE LNDWLTKTEE RTRKMEKEPL GPDIEDLKRQ VQQHKVLQED LEQEQVRVNS LTHMVVVVDE SSGDHATAAL EEQLKVLGDR WANICRWTED RWVLLQDILL KWQRFTEEQC LFSAWLSEKE DAVNKIHTTG FKDQSEVLSN LQKLAVLKTD LEKKKQTMDK LCSLNQDLLS ALKNTVVAHK MEAWLDNFAQ RWDNLVQKLE KSSAQISQAV TTTQPSLTQT TVMETVTMVT TREHILVKHA QEELPPPPPQ KKRQIIVDSE IRKRLDVDIT ELHSWITRSE AVLQSPEFAI YRKEGNFSDL KEKVNAIERE KAEKFRKLQD ASRSAQALVE QMVNEGVNAD SIKQASEQLN SRWIEFCQLL SERLNWLEYQ NNIITFYNQL QQLEQMTTTA ENWLKTQPTT TSEPTAIKSQ LKICKDEINR LSALQPQIER LKIQSIALKE KGQGPMFLDA DFVAFTNHFN QVFADVQARE KELQTIFDSL PPMRYQETMS TILTWIQQSE TKLSIPQVTV TEYDIMEQRL GELQALQSSL QEQQNGLNYL STTVKEMSKK APLSDISRKY QSEFEEIEGR WKKLSSQLVE HCQKLEEQMA KLRKIQNHIK TLKKWITEVD VFLKEEWPAL GDSEILKRQL KQCRLLVNDI QTIQPSLNSV NEGAQKMKNE AEPEFAGRLE TELRELNTQW DYMCRQVYAR KEALKGGLDK TVSLQKDLSE MHEWMTQAEE EYLERDFEYK TPDELQTAVE EMKRAKEEAQ QKEAKVKLLT ESVNSVIAQA PPAAQEALKK ELDTLTTNYQ WLCTRLNGKC KTLEEVWACW HELLSYLEKA NKWLSEVEVK LKTTENISGG AEEIAEVLDS LENLMQHSED NPNQIRILAQ TLTDGGVMDE LINEELETFN SRWRELHEEA VRRQKLLEQS IQSAQEIEKS LHLIQESLSS IDKQLAAYIA DKVDAAQMPQ EAQKIQSDLT SHEISLEEMK KHNQGKETAQ RVLSQIDVAQ KKLQDVSMKF RLFQKPANFE QRLQESKMIL DEVKMHLPAL ETKSVEQEVV QSQLNHCVNL YKSLSEVKSE VEMVIKTGRQ IVQKKQTENP KELDERVTAL KLHYNELGAK VTERKQQLEK CLKLSRKMRK EMNALTEWLA ATDMELTKRS AVEGMPSNLD SEVAWGKATQ KEIEKQKVHL KSVTEVGEAL KTVLGKKEML VEDKLSLLNS NWIAVTSRAE EWLNLLLEYQ KHMETFDQNV DYITNWIIQA DALLDESEKK KPQQKEDILK RLKAEMNDIR PKVDSTRDQA ANLMANRGDH CRKVVEPKIS ELNHRFAAIS HRIKTGKASI PLKELEQFNS DIQKLLEPLE AEIQQGVNLK EEDFNKDMSE DNEGTVKELL QRGDNLQQRI TDERKREEIK IKQQLLQTKH NALKDLRSQR RKKALEISHQ WYQYKRQADD LLKCLDDIEK KLASLPEPRD ERKIKEIDRE LQKKKEELNA VRRQAEGLSE DGAAMAVEPT QIQLSKRWRE IESKFAQFRR LNFAQIHTVH EESVVAMTED MPLEISYVPS TYLTEITHVS QALSEVEELL NAPDLCAQDF EDLFKQEESL KNIKDSLQQI SGRIDIIHNK KTAALHSATP AERAKLQEAL SRLDFQWERV NNMYKDRQGR FDRSVEKWRR FHYDMKILNQ WLTEAEQFLK KTQIPENWEH AKYKWYLKEL QDGIGQRQSV VRVLNATGEE IIQQSSKTDA SILQEKLGSL NLRWQEVCKQ LAERKKRLEE QKNILSEFQR DVNEFVLWLE EADNVANIPL EPGNEQQLKE KLEQVKLLAE ELPLRQGILK QLNETGGTVL VSAPLSPEEQ DKLENKLKQT NLQWIKVSRN LPEKQEEIEA HVKDLGQLEE QLNHLLLWLS PIRNQLEIYN QPNQTGPFDI KEIEVAVQAK QPDVEGILSK GQHLYKEKPA TQPAKRKLED LSSDWKVVTQ LLQELRAKQP GPAPGLTTVR APPSQTVTLV TQPAVTKETA ISKLEMPSSL LLEVPALADF NRAWTELTDW LSLLDRVIKS QRVMVGDLED INEMIIKQKA TLQDLEQRRP QLEELITAAQ NLKNKTSNQE ARTIITDRIE RIQSQWDEVQ EHLQNRRLQL TEMLKDSTQW LEAKEEAEQV LGQARAKLES WKEAPYTVDA IQKKITETKQ LAKDLRQWQI NVDVANDLAL KLLRDYSADD TRKVHMITEN INASWASIHK RLSEREAALE ETHRLLQQFP LDLEKFLAWL TEAETTANVL QDATHKERLL EDSKGVRELM KQWQDLQGEI EAHTDIYHNL DENGQKVLRS LEGSDDAALL QRRLDNMNFK WSELRKKSLN IRSHLEASSD QWKRLHLSLQ ELLVWLQLKD DELSRQAPIG GDFPAVQKQN DVHRAFKREL KTKEPVIMST LETVRIFLTE QPLEGLEKLY QEPRELPPEE RAQNVTRLLR KQAEEVNTQW EKLNVHSADW QRKIDEALER LQELQEATDE LDLKLRQAEV IKGSWQPVGD LLIDSLQDHL EKVKALRGEI TPLKENVSYV NDLARQLTTL GIQLSPYNLN TLEDLNTRWK LLQVAIEDRI RQLHEAHRDF GPASQHFLST SVQGPWERAI SPNKVPYYIN HETQTTCWDH PKMTELYQSL ADLNNVRFSA YRTAMKLRRL QKALCLDLLS LSAACDALDQ HNLKQNDQPM DILQVINCLT TIYDRLEQEH NNLVNVPLCV DMCLNWLLNV YDTGRTGRIR VLSFKTGIIS LCKAHLEDKY RYLFKQVASS TGFCDQRRLG LLLHDSIQIP RQLGEVASFG GSNIEPSVRS CFQFANNKPE IEAALFLDWM RLEPQSMVWL PVLHRVAAAE TAKHQAKCNI CKECPIIGFR YRSLKHFNYD ICQSCFFSGR VAKGHKMHYP MVEYCTPTTS GEDVRDFAKV LKNKFRTKRY FAKHPRMGYL PVQTVLEGDN METPVTLINF WPVDSAPASS PQLSHDDTHS RIEHYASRLK KMENSNGSYL NDSISPNESI DDEHLLIQHY WRSLNQESPL SQPRSPAQIL ISLESEERGE LERILADLEG RNRNLQAEYD RLKQQHEHKG LSPLPSPPEM MPTSPQSPRD AELIAEAKLL RQHKGRLEAR MQILEDHNKQ LESQLHRLRQ LLEQPQAEAK VNGTTVSSPS TSLQRSDSSQ PMLLRVVGSQ TSESMGEEDL LSPPQDTSTG LEEVMEQLNH SFPSSRGRNT PGKPMREDTM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.

Gene References into Functions

The deletion was confirmed with conventional PCR and Sanger sequencing. This mutation is in a secondary DMD gene hotspot area distinct from the one identified earlier at the 5' donor splice site of intron 50 in the CKCS breed. PMID:28028563
Partial myostatin loss may exaggerate selective muscle hypertrophy or atrophy/hypoplasia in dystrophin-deficient dogs and worsen contractures. PMID:27047655
a chromosome inversion disrupting the DMD and RPGR genes is the causative mutation for X-linked muscular dystrophy in the Japanese Spitz breed PMID:25644216
Data indicate that dystrophin affects caveolar integrity and airway smooth muscle contraction. PMID:25054970
These data are consistent with the notion that altered N-glycolylneuraminic acid and cytotoxic T cell carbohydrate expression may modify severity of muscular dystrophy resulting from dystrophin deficiency in dogs and humans. PMID:24505439
These data underscore the muscle-specific responses to dystrophin deficiency and the potential deleterious effects of differential muscle involvement. PMID:24160322
Data indicate extensive expression of skipped RNA and dystrophin protein in the treated myocardium. PMID:22551778
eccentric contractions induce greater-than-normal isometric torque drop in dystrophin-deficient dog muscles PMID:20544944
Stem cell transplantation does not improvse dystrophin expression in Duchenne muscular dystrophy. PMID:15328150
Dystrophin depletion closely coincided with generally depressed transcription and translation in acute ischemic myocardial injury and reperfusion. PMID:15850566
Selective vacuolar degeneration of Purkinje fibers was found in the early stages of dystrophin deficiency PMID:18458171

Hide All

Subcellular Location

Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell junction, synapse, postsynaptic cell membrane.

Database Links

UNIGENE: Cfa.3738

KEGG: cfa:606758

STRING: 9615.ENSCAFP00000031637

Promotions