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Recombinant Dictyostelium discoideum Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (pteN), partial

Recombinant Dictyostelium discoideum Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (pteN), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

pteN

Purity

>85% (SDS-PAGE)

Target Names

pteN

Uniprot NO.

Q8T9S7

Alternative Names

pteN; ptenA; DDB_G0286557; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67; Pten 3-phosphoinositide phosphatase alpha

Species

Dictyostelium discoideum (Slime mold)

Source

Yeast

Expression Region

1-533

Target Protein Sequence

MSNLLRVAVS KQKRRYQKNG YDLDLAYITD NIVAMGFPSE KVEGVFRNPM KDVQRFLDQY HKDHFKVYNL CSERVYDHSK FYGRVGYYPF DDHNAPQFEM IDAFCRDVDA WMKEDSKNIA VIHCKAGKGR TGLMICCWLM YCGMWKNTED SLRFYAALRT YNQKGVTIPS QIRYVGYFGR SIRESIKYVP RNVTLKKIVL RPLPKEINLS EVQFNISVGK NCVFNSKEHN MNVVISKKKK TVVDKNKKDP KKKLTKENSE KNIDSQQQQQ SQSSLSQSQQ GQSSPNMQSL SASGTISSGS NVGTVNGNTL HQLGGSQFSL SDLADGNTIG NDEYISFEIG ALSLAGDIRI EFTNKQDDRM FMFWVNTSFV QQLEIIPKSG LDKAHKDKNH KAFPEDFHVE LTFDQLDQQQ SHTTVVASAE EQTNNQHYPQ SSNNVATSSS HHDNITVVAS DAPQNNNNNN NLNSSNSNNA TTTTTKNNIS LASSQSNPVQ QESNPSTTTQ VSEENSAPKV EAEKIENSNA SANDSETSSN SSS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate. Negative regulator of PI3K chemotaxis pathways. Overexpression leads to a suppression of a PI3K-dependent activation of pkbA, and these cells exhibit chemotaxis defects consistent with a reduction in PI3K activity.

Gene References into Functions

Asymmetric Ras activation in turning protrusions underscores that gradients can be sensed locally and translated into orientation. Different to cells of normal size, the polarity of large cells is not linked to an increasing front-to-tail gradient of the PIP3-phosphatase PTEN. PMID:27505897
PTEN is involved in the regulation of lateral pseudopod formation, a process essential for locomotion and chemotaxis, but not for directional sensing. PMID:17623773
Studied PTEN and show positively charged residues located in the calpha2 helix of the C2 domain are necessary for the membrane localization of PTEN via stable electrostatic interactions in Dictyostelium discoideum. PMID:25211206

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Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Cytoplasm, cell cortex. Note=Found uniformly on the plasma membrane in unstimulated cells. In response to chemoattractant stimulation, there is a rapid and transient release from the plasma membrane. Constitutively localized in the cortex of polarized cells.

Database Links

KEGG: ddi:DDB_G0286557

STRING: 44689.DDB0191093

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