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Recombinant Dictyostelium discoideum DNA topoisomerase 1 (top1), partial

Recombinant Dictyostelium discoideum DNA topoisomerase 1 (top1), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

top1

Purity

>85% (SDS-PAGE)

Target Names

top1

Uniprot NO.

Q54RC3

Alternative Names

top1; DDB_G0283205; DNA topoisomerase 1; EC 5.6.2.1; DNA topoisomerase I

Species

Dictyostelium discoideum (Slime mold)

Source

Yeast

Expression Region

1-893

Target Protein Sequence

MEVKQEILTP MKVEKTNGNV PTPPPTTTTT TVMDDDLFDD EASPPQPNKK LKAENGSIKK LTPTPKPAPV TPKPTAAVNG KKPIPKKKPV DSSSDSDSSD SSSDSDSDSS DSDSDSSSSD SSSSDSDVPK KKPISKIPNK PSGTVSKTSK NSPKTTTTTT TTTTTVKSKP APKVKPEQSS SDDSSSDSSD DSSDDSSSDS SSEEEKPKKK PSKKPAAVKK EITVKKETTT TTKKTPVKKE VEVKKETKTT TTKKVKKEES DDDGEDEKTT TKKKTTTRKR KLKEEEEGEE EEEEGVNKWW DKEDDEDDGT KWTTLIHNGP VFPPAYVPHG IKFMYDGKVV KLTPKQEEVA TFYANYLETD HVKKQAFKDN FFKEFKGLLT SEQKEIVKKM DKCDFSHIHT HLKTKKEQRK ARSKEEKDAE TAEKKKIQEK HGFATIDGFK EKVGNYMIEP PGLFLGRGQH PKTGMLKQRI MPSDVTLNIG EGEVIPPSPI PGEKWKEIVH NNTVTWLAFW RESVNGGFKY VWLAPSSKIK GQADRKKFEV ARGLKNCIGD IRKGYQKSFN DESTEKRQLA VALYLIDFLA LRVGNEKGED QADTVGCCSL RVEHIKLESN NTITLDFLGK DSMRYLNTVQ IREDAYKVLK TFVKGKKPTD LLFDSLTVTQ LNNHLKKQMT GLSAKVFRTY NASITLQKEL DKMDTKEFQS IDEKILFYNR CNREVAILCN HQKAPPKNLA ETMSKIDEKI QELRDLQDLC RIKIGKTPKS QQEDIESREA ERLPKLIQLA ITNKKDKDKE KTDKEISFDV KAAFEKSRTL PDAKGKIKEK IAKYEEQIKK QEILKTGKDE LKTVALGTSK INYLDPRITV AWCKKNKVPV DKIFTKSLRD KFPWSDVGTD YIF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. TThe free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Subcellular Location

Nucleus.

Protein Families

Type IB topoisomerase family

Database Links

KEGG: ddi:DDB_G0283205

STRING: 44689.DDB0234191

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