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Recombinant Daucus carota DNA topoisomerase 1 (TOP1), partial

Recombinant Daucus carota DNA topoisomerase 1 (TOP1), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

TOP1

Uniprot NO.

P93119

Alternative Names

TOP1; DNA topoisomerase 1; EC 5.6.2.1; DNA topoisomerase I

Species

Daucus carota (Wild carrot)

Source

Yeast

Expression Region

1-790

Target Protein Sequence

MKSNPGITVI KQNASNVMEK TLKEEGQSRS NSEDSDDDKP LSHKLSSGAL NGNSHHIRMG SNLSCPSPYT SPKPRIIKGP EDEMLLSSEL QLNAGASNAE SSDSDESKPL ASELEDPSSS LNKRPLIQPK NSDPSPSKKA KLSEPSAPTN RKLKTAEQEE AAADDDPSIS NRNKKSTTPA SKVSDKKKRP DVSASVNKVD FSKSLKVPLV LVKGRNGQPL VHNGVIFPPL YKPHGVKMLY RENPVDLTPE QEEVATMFAV MLETEYMTKP KFRENFMSDW RKILGEKHII QNLEDCDFTP IYEWHQREKE KKKQMSTDEK KAIKEGKDET RKEKYMWAVV DGMSREKVGN FRVGTTRVVQ RSWRASKDPQ VKKNVYSQID ITINIGKDAP IPEPPIPGER WKEIRHDNTV TWLAFWNDPI NPKEFKYVFL AASSSLKGKV TREKYEKSRK LKDYIEGIRA AAYTKDFASK DSKKRQIAVA TYLIDKLALR AGNEKDDDEA DTVGCCTLKV ENVETKRPNI LKFDFLGKDS IRYQNEVEVE PRVFSAIEQF RSGKEGGDDL FDQLDTSKLN AHLKELMPGL TAKVFRTYNA SITLDEMVRC KLLSRETKGG DVAEKVVVYQ HANKEVAIIC NHQRTVSKSH SAQMVRLNEK IEELKGLLKE LQEDLTRVNK GKPPLKNSDG KPKRNLNLKR YKGKLLKLIQ KLRKWSETRR LSVTKKIAQT NTKIEKMERD KETKEDLKTV ALGTSKINYL DPRITVAWCK RHDVPIEKIF NKSLLAKFAW AMDVVLIFRF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Subcellular Location

Nucleus.

Protein Families

Type IB topoisomerase family

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