Recombinant Danio rerio Nestin (nes), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

nes

Uniprot NO.

P86839

Alternative Names

nes; Nestin

Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Source

Yeast

Expression Region

1-1265

Target Protein Sequence

MELLGVRQPF GPQFQEEKYQ MLELNHRLES YLGRVKLLEE ENKLLREEIH TLKSSRDPAG QRKAQEEALS QARRMMEEAW RKKDCVELEV ENLMEDMEKV SFQRKKVKTA QAEAQRKLTE SRKELEEERR AQIWLREKVG QLEKDLMLQM QVHQENMETM QASLKQTKQV LMAPQRVQAT SIPDLGQEYG YKAAQAWQEA ANNYQKQVGR LEESLNQTKA NMTKIYQEKR ESQRQVQHLA KELESTRIKR QMLEKHAMQQ REEQKEELQH LQAQVNTLEL EKDCLGQQID SLMLDRQHLL QVKMSLGLEV ATYRALLDSE GLRIDRPTPN KTSSTVFLDA LSKPTGIQST SQTTAASCLL SSSVSTSHRS IASSRSLLTS AAPSWTLTRG TPQRPPSSTS MTEKTEDHIS EETKRSAEES VDQLQQEKVQ QDWTLESTLP KTSAEPKPEL QPEEIKVEDE ANEPQQAHMV ESKTDESELT SVPAEQQGSM SQTPETKSWA GHFNDPAEVS EDGKDEDTEV SVEMARISHA PKVAWEEIKS AVEDEKDDAS EMDVRSEIIS ESHTFAYGDA ENDSNTLTSS HISQNTNALA SSFLEQGTLD SASYFGYEAT NENLMKEEEL DNVSNISEEV TEQLNSETEA AIDSINEWDR QEESEPENET KVMTSYFEVE EGEMSINTDT KDKTEDDIER EELEVTEREI LVQSAGGMLG VDLPNDTDHQ DEHNLPQIEL NEDQSEAEEE NQKEKQCDED DSPNISASLK TDPGEGDSYS QEHTLADTRP LIRYKSDEET SHHIGETSDS EDEKERIDQG HLNEKRFNTM EDLTEEPDME VTGKMRLEDL VSNEEAAADD VASVMFQKVS GDHESLDVVV QESERKGNLE KEDSGDADNM RDNKEEDVSV FEQNENQQLT EHQHVHTEQV EDKPVHSHET QEHVNTEFSS TFSERSQQEQ LEESSLTMFE DEAATKEAED SDVSMHTNID LIDSRSLESE INGQPDIMTS DMANSECNSS EDESPNASQC FQNTSLLAAA TPNEQPLTFT NEVSKADYVS DNNDVPEEVL SEEKSTDEPK ASQIDDLENF NIWGESTSIS DAAQTTHASM DEHSSISPVN ENLESSNKIP SVAENIFGHF EEHLERSVES FPEKEVTVMD FENNDLGEEF QSKQMKSEIH SFFSTSLKQD FWSEGKMEMA ATYDPAKTED LNQAMVFGEE WREIGVQSAN GDTKEEMEIL KIRDDKQKDG QPAQSKIVQS DDSADEGDSW SSGDE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. Required for brain and eye development.

Gene References into Functions

Morphine increases GFP in nestin:GFP embryos and overexpresses the NSC marker Nestin. Morphine also exerts a hyperacetylation effect on H3K27 and decreases DNA methylation in a region 18 Kb upstream of the nestin transcription starting site. A binding site for the transcription factor complex Sox2/Oct4/Nanog was predicted. PMID:29111275
Nestin gene may serve as a marker for stem cells and proliferating precursors in the developing embryonic nervous system as well as in the postembryonic brain[Nestin] PMID:17651502
Results suggest that nestin is essential for brain and eye development probably through control of progenitor cell apoptosis. PMID:20174467

Hide All

Protein Families

Intermediate filament family

Tissue Specificity

Widely expressed throughout the developing nervous system at 24 hours post-fertilization (hpf). As development progresses, expression becomes restricted to proliferative zones of the developing and postembryonic central nervous system. In the peripheral n

Database Links

UNIGENE: Dr.151468

KEGG: dre:100150939

STRING: 7955.ENSDARP00000112046

Promotions