Recombinant Macaca fascicularis Interleukin-4 (IL4)

The expression of recombinant Macaca fascicularis IL4 protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the yeast, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis tag sequence to the designated sequence encoding the 25-153aa of the Macaca fascicularis IL4 protein. This N-terminal 6xHis-tagged recombinant Macaca fascicularis IL4 protein is also characterized by high purity, >90%. Under SDS-PAGE condition, this recombinant IL4 protein migrated to the bands of about 30 kDa and 17 kDa molecular weight, corresponding to the glycosylated and deglycosylated forms, respectively.

As the B cell-stimulatory factor-1, IL-4 is mainly produced by activated T cells. In T-cells, IL-4 induces the differentiation of naïve CD4 T cells into Th2 cells, in B cells, IL-4 drives the immunoglobulin (Ig) class switch to IgG1 and IgE, and in macrophages, IL-4 and IL-13 induce alternative macrophage activation. It has been well-documented that it plays a major role in hormone control and adaptive immunity. In addition, IL-4 a key regulator in inflammation response and wound repair. Many papers give insights into the functional formation of IL-4. Studies found that IL-4 coordinates the Th2-type immune response in inflammatory diseases such as asthma. IL-27 can inhibit the development of both Th2 and Th1. Prolonged residence of an albumin–IL-4-fusion protein in secondary lymphoid organs ameliorates experimental autoimmune encephalomyelitis. IL-4 absence could trigger the distinct pathways in apical periodontitis development. Association between IL-4 gene polymorphisms and IL-4 serum levels in patients with allergic rhinitis. Status of IL‐4 and IL‐10 driven markers in experimental models of visceral leishmaniasis.