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Recombinant Clostridium difficile Proline--tRNA ligase 1 (proS1), partial

Recombinant Clostridium difficile Proline--tRNA ligase 1 (proS1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Abbreviation

proS1

Purity

>85% (SDS-PAGE)

Target Names

proS1

Uniprot NO.

Q18CD2

Alternative Names

proS1; CD630_00490; Proline--tRNA ligase 1; EC 6.1.1.15; Prolyl-tRNA synthetase 1; ProRS 1

Species

Peptoclostridium difficile (strain 630) (Clostridium difficile)

Source

Yeast

Expression Region

1-571

Target Protein Sequence

MKMSKMFMPT LKEIPADAEI TSHQLMVRSG MIKKMTSGVY NQLPMGLRVF KKIEQIIREE LNKKDCQEIL CAALLPSELW KESGRWTAMG EEMFRLKDRT EREYCLGPTH EEAFTDIIRQ EITSYKQLPL NLYQIQVKYR DERRPRFGVM RTKTFTMKDA YSFDVDDKGL DKSYQDMFDA YVSIFDRCGL ENSPVQADSG AIGGSTSAEF MVKSEVGEDE VVFCSGCDYA ANVERAESCN LASQKEEMKE LEEVHTPGAA TIKELEEFLK TSPDKFAKTL VYEADGKTVV VVVRGDREVN EIKVSNAIGS VIEFALATDD VVRKVTNAEV GFAGPIGINA DYVFIDKEIV EQRNIVVGAN KTEYHIKNAN YGRDFEGIVG DFRNVQEGDK CIVCGKPLEI ARGVEVGHIF KLGTKYSESM NANFIDKDGK SKPIVMGCYG IGVERTAAAI IEQHNDEKGI IWPLSVAPYH VVIVPANMKN EEQISIAENI YNDLQAMGVE VLLDDRDERI GVKFNDSELI GIPMRITVGK NINEGKVEFK LRHKEDKEII DIEEINEKVK AEFIRNNVRL G
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.

Subcellular Location

Cytoplasm.

Protein Families

Class-II aminoacyl-tRNA synthetase family, ProS type 1 subfamily

Database Links

KEGG: cdf:CD630_00490

STRING: 272563.CD0049

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