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Recombinant Chicken Serine/threonine-protein kinase B-raf (BRAF), partial

Recombinant Chicken Serine/threonine-protein kinase B-raf (BRAF), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

BRAF

Uniprot NO.

Q04982

Alternative Names

BRAF; RMIL; Serine/threonine-protein kinase B-raf; EC 2.7.11.1; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; Serine/threonine-protein kinase Rmil

Species

Gallus gallus (Chicken)

Source

Yeast

Expression Region

1-806

Target Protein Sequence

MAALSSGSSA EGASLFNGDM EPEPPPPVLG ACYAGSGGGD PAIPEEVWNI KQMIKLTQEH IEALLDKFGG EHNPPSIYLE AYEEYTSKLD ALQQREQQLL ESMGNGTDFS VSSSASTDTV ASSSSSSLSV APSSLSVYQN PTDMSRNNPK SPQKPIVRVF LPNKQRTVVP ARCGVTVRDS LKKALMMRGL IPECCAVYRI QDGEKKPIGW DTDISWLTGE ELHVEVLENV PLTTHNFVRK TFFTLAFCDF CRKLLFQGFR CQTCGYKFHQ RCSTEVPLMC VNYDQLDLLF VSKFFEHHPI SQEETTLGET TPASGSYPSV PPSDSVGPPI LPSPSPSKSI PIPQPFRPAD EDHRNQFGQR DRSSSAPNVH INTIEPVNID DLIRDQGVRG EGAPLNQLMR CLRKYQSRTP SPLLHSVPSE IVFDFEPGPV FRGSTAGLSA TPPASLPGSL TNVKALQKSP GPQRERKSSS SSEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIHRSASE PSLNRAGFQT EDFSLYACAS PKTPIQAGGY GGFPVH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Protein kinase involved in the activation of the MAP signaling cascade. May play a role in transducing specific signals in neural cells.

Gene References into Functions

BRAF alternative splicing is differentially regulated in avian, rodent and primates. Exon 9b is present in vertebrates but exon 8b is present only in eutherians. PMID:21203559
Neutralizing mutations of D447/8, S446 & S447, or uncoupling of B-Raf from Ras-GTP, reduce chicken B-Raf actvity in PC12 differentiation. S365 is a 14-3-3 binding site. B-Raf(V600E) cannot be inhibited by blocking S446 phosphorylation or Ras activation. PMID:16702958
Inducible gene deletion of the c-Rmil/B-raf gene in chicken DT40 B cells revealed that B-Raf is required for the maintenance of basal ERK activity as well as for a rapid and sustained ERK activation following B cell antigen receptor (BCR) activation. PMID:12411479

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Subcellular Location

Nucleus. Cytoplasm. Cell membrane.

Protein Families

Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily

Tissue Specificity

Expressed preferentially in neural tissue.

Database Links

UNIGENE: Gga.719

KEGG: gga:396239

STRING: 9031.ENSGALP00000020950

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