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Recombinant Chicken Retinoid isomerohydrolase (RPE65), partial

Recombinant Chicken Retinoid isomerohydrolase (RPE65), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Target Background

Product Details

Abbreviation

RPE65

Purity

>85% (SDS-PAGE)

Target Names

RPE65

Uniprot NO.

Q9YGX2

Alternative Names

RPE65; Retinoid isomerohydrolase; EC 3.1.1.64; All-trans-retinyl-palmitate hydrolase; Lutein isomerase; Meso-zeaxanthin isomerase; EC 5.3.3.22; Retinal pigment epithelium-specific 65 kDa protein; Retinol isomerase

Species

Gallus gallus (Chicken)

Source

Yeast

Expression Region

1-533

Target Protein Sequence

MYSQVEHPAG GYKKLFETVE ELSSPVTAHV TGRIPTWLRG SLLRCGPGLF EVGAEPFYHL FDGQALLHKF DFKEGHVTYH RRFVRTDAYV RAMTEKRIVI TEFGTYAYPD PCKNIFSRFF SYFKGVEVTD NALVNVYPVG EDYYACTETN FITKINPDTL ETIKQVDLCK YVSVNGATAH PHVENDGTVY NIGNCFGKNF SLAYNIIRIP PLQADKEDPM NKSEVVVQFP CSDRFKPSYV HSFGLTPNYI VFVETPVKIN LLKFLSSWSL WGANYMDCFE SNETMGVWLH VAEKKKGRLL NIKYRTSAFN LFHHINTFED NGFLIVDLCT WKGFEFVYNY LYLANLRANW DEVKKQAEKA PQPEARRYVL PLRIDKADTG KNLVTLPYTT ATATLRSDET VWLEPEVIFS GPRHAFEFPQ INYKKYGGKP YTYTYGLGLN HFVPDRLCKL NVKTKETWVW QEPDSYPSEP IFVSHPDALE EDDGVVLSIV ISPGSGPKPA YLLILNAKDM SEVARAEVEV NIPVTFHGLF KRA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.

Gene References into Functions

RPE65 undergoes conformational changes in secondary and tertiary structures upon binding with the phospholipid membrane. These conformational rearrangements facilitate restoration of a catalytically active state of RPE65. PMID:21446919
Rpe65 isomerase associates with membranes through an electrostatic interaction with acidic phospholipid headgroups. PMID:19892706
RPE65 from the cone-dominant chicken RPE possesses significantly higher specific retinol isomerohydrolase activity, when compared with RPE65 from rod-dominant species PMID:18216020
Results provide conclusive evidence that RPE65 is the isomerohydrolase of the visual cycle. PMID:19490105