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Recombinant Candida albicans tRNA ligase (LIG1), partial

Recombinant Candida albicans tRNA ligase (LIG1), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

LIG1

Uniprot NO.

P43075

Alternative Names

LIG1; RLG1; TRL1; CAALFM_C702060WA; CaJ7.0238; CaO19.13864; CaO19.6511; tRNA ligase; EC 6.5.1.3

Species

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Source

Yeast

Expression Region

1-832

Target Protein Sequence

MKDSQSDIIE LCNKLNEATK LKRNGKSIKL TNFVSNTQIK LDSWKFLEWD YGKPSVQLPI QARGLFTLNN DTIAVRGYDK FFNVEEKPFT KETNLKTSTH GPYEVTLKEN GCIIFISGLS TGDIVVCSKH STGDRIDDNE SDKTTTATAT ATAPTRNHAK QGEFELLQQF DGDQQKVKQL AHYLYENNLT VVAELCDDEF EEHVLPYPKD KSGLYVHGLN YNTITFKTLP MDQVLQFAKE WGFKYVSYLT YDNADELFKF LHKCSETGTY NGREIEGFVI RCHRQSHTNG DTDGDCFFFK YKFEQPYLLY RQFREVTKQL LNGTPINSIK IKKNKPITKK YLQFVEKLFE QEPEIARNFE NGFDIIKVRQ LFLQSLNETN GMNLLSIDSE LSDQLKNLAL ANGNEGLSTT TKYIFVPIAT IGCGKTTVFN TLNNLFPQWT HIQNDNISKK AKLKICDLTL LALEDDDQSV VLFDRNNSAS RERRQIFTTI DQKRDEHLDD TVDLKYIAIN FIPEDLSEEE LWDITYNRVI QRGDNHQSIK SQSDENLVES VMKGFIQRYQ PINTSRSPDD QFDHVIHLKL SKDENSSKSS LENVRIIIDD LVQNFPDLIK EKPADELINE CFQKALDYKP TFVKNMTANT IKKDPTYYGI AMHYSSILEN LEIVSHNEHF QNIKSHIQTE FHVTLGHIAS SKQDKAGRVK WKKLVKTLGK GDPNKPKSAL KFFADVKLLQ IVINTDKLAC IKVEILKIYD TNDVLQSEIE PINKQLHITI GCIPPATAVE SNITLEELYD NPDEQELKPD GTYKCGDDTL HVFNFDNPDL KLFSQQLFVA YQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

One of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity requires three enzymatic activities: phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP-dependent reaction.

Gene References into Functions