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Recombinant Bovine cGMP-dependent protein kinase 1 (PRKG1), partial

Recombinant Bovine cGMP-dependent protein kinase 1 (PRKG1), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PRKG1

Uniprot NO.

P00516

Alternative Names

PRKG1; PRKG1B; PRKGR1A; PRKGR1BcGMP-dependent protein kinase 1; cGK 1; cGK1; EC 2.7.11.12; cGMP-dependent protein kinase I; cGKI

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

2-671

Target Protein Sequence

SELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP STHIGPRTTR AQGISAEPQT YRSFHDLRQA FRKFTKSERS KDLIKEAILD NDFMKNLELS QIQEIVDCMY PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV TKEGVKLCTM GPGKVFGELA ILYNCTRTAT VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT EYMEFLKSVP TFQSLPEEIL SKLADVLEET HYENGEYIIR QGARGDTFFI ISKGKVNVTR EDSPNEDPVF LRTLGKGDWF GEKALQGEDV RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA FFANLKLSDF NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF IVRLYRTFKD SKYLYMLMEA CLGGELWTIL RDRGSFEDST TRFYTACVVE AFAYLHSKGI IYRDLKPENL ILDHRGYAKL VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD YWSLGILMYE LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD SFPEDNDEPP PDDNSGWDID F
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates IRAG1 and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.

Gene References into Functions

NADPH regulation of PKG1-alpha dimerization may contribute to vascular responses to hypoxia that are associated with changes in NADPH redox. PMID:23709600
The data suggest that PKG-I expression is subjected to post-transcriptional regulation in VSMC through the 3'UTR of its mRNA. PMID:21292805
Report roles for redox mechanisms controlling protein kinase G in pulmonary and coronary artery responses to hypoxia. PMID:21926339
Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication PMID:21893290
purified cGMP kinase signaling complex contained alpha-actin, calponin H1, and phospholamban PMID:12480535
studies suggest that cross-activation of PKA in response to iNOS expression by inflammatory mediators downregulates cGMP-dependent protein kinase expression in bovine aortic vascular smooth muscle cells PMID:14985234
Demonstrate a novel PKG-dependent pathway by which the Rac1/Pak1 pathway is activated. PMID:15212766
cGMP-dependent protein kinase expression is regulated by soluble guanylyl cyclase in vascular smooth muscle cells PMID:15337747
cGMP-dependent protein kinase Ibeta binds to TFII-I and IRAG through a common interaction motif PMID:16166082
cGMP binding destabilizes the N-terminus of cGMP-dependent protein kinase I alpha isozyme at the hinge region, centered around residue 77, while the C-terminus is protected from degradation. PMID:17403045

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Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily

Tissue Specificity

High concentrations are detected in various smooth muscle: lung, rumen, trachea, aorta, uterus and stomach. Isoform alpha is expressed predominantly in heart, cerebellum and lung, whereas the beta isoform is expressed in high concentrations in trachea, ao

Database Links

UNIGENE: Bt.63035

KEGG: bta:282004

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