Recombinant Bovine Vimentin (VIM)

Code
MSDS	MSDS
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

VIM

Uniprot NO.

P48616

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

2-466

Target Protein Sequence

STRSVSSSS YRRMFGGPGT ASRPSSTRSY VTTSTRTYSL GSALRPTTSR TLYTSSPGGV YATRSSAVRL RSGVPGVRLL QDSVDFSLAD AINTEFKNTR TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE STLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHDE EIQELQAQIQ EQHVQIDMDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE AANRNNDALR QAKQESNEYR RQVQTLTCEV DALKGTNESL ERQMREMEEN FSVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR DGQVINETSQ HHDDLE

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Gene References into Functions

Immunocytochemistry for Vimentin detection in nuclei of IVF and NT bovine embryos PMID:15483223
These results indicate that the inner mass differentiates dynamically in blastocysts, as reflected by the expression of vimentin; higher vimentin expression may reflect the higher developmental competence of embryos. PMID:19019059
an intact vimentin intermediate filament network contributes to the maintenance of the chondrocyte phenotype PMID:16876394

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.

Protein Families

Intermediate filament family

Database Links

UNIGENE: Bt.11149

KEGG: bta:280955

STRING: 9913.ENSBTAP00000024572

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