Recombinant Bovine Prothrombin (F2), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Uniprot NO.

P00735

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

44-625

Target Protein Sequence

ANKGFLE EVRKGNLERE CLEEPCSREE AFEALESLSA TDAFWAKYTA CESARNPREK LNECLEGNCA EGVGMNYRGN VSVTRSGIEC QLWRSRYPHK PEINSTTHPG ADLRENFCRN PDGSITGPWC YTTSPTLRRE ECSVPVCGQD RVTVEVIPRS GGSTTSQSPL LETCVPDRGR EYRGRLAVTT SGSRCLAWSS EQAKALSKDQ DFNPAVPLAE NFCRNPDGDE EGAWCYVADQ PGDFEYCDLN YCEEPVDGDL GDRLGEDPDP DAAIEGRTSE DHFQPFFNEK TFGAGEADCG LRPLFEKKQV QDQTEKELFE SYIEGRIVEG QDAEVGLSPW QVMLFRKSPQ ELLCGASLIS DRWVLTAAHC LLYPPWDKNF TVDDLLVRIG KHSRTRYERK VEKISMLDKI YIHPRYNWKE NLDRDIALLK LKRPIELSDY IHPVCLPDKQ TAAKLLHAGF KGRVTGWGNR RETWTTSVAE VQPSVLQVVN LPLVERPVCK ASTRIRITDN MFCAGYKPGE GKRGDACEGD SGGPFVMKSP YNNRWYQMGI VSWGEGCDRD GKYGFYTHVF RLKKWIQKVI DRLGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Gene References into Functions

the weaker rapid interaction between prothrombin and membranes is the most important in vivo when considering the activation of prothrombin at the cell surface. PMID:23812842
novel pyranosic sulfated arabinan Ab1 exerts its anticoagulant activity on thrombin by a mechanism different from those found previously for other sulfated polysaccharides and glycosaminoglycans PMID:23161548
Exogenous delivery of thrombin enhances microvascular collateral development in response to ischemic insult, and accelerates tissue reperfusion. PMID:21954923
the signaling pathways of MAPK, MSK1, and NF-kappaB play important roles in thrombin-induced iNOS expression in alveolar macrophages PMID:22004609
High glucose enhances smooth muscle cell responsiveness to thrombin through transcriptional upregulation of PAR-4, mediated via PKC and NFkappaB. PMID:21164077
Thrombin induces a sustained contraction in the normal pulmonary artery, by activating PAR(1) and thereby increasing the sensitivity of the myofilament to Ca(2+). PMID:20128804
kinetic and structural analysis of interactions between thrombin and the Factor XIII activation peptide PMID:12669118
analysis of staphylocoagulase-mediated bovine prothrombin activation PMID:16230338
crystal structure PMID:18045235
thrombin and factor Xa diffusion along the heparin molecule explains the effects of extended heparin chain lengths PMID:18045667
A comparison of recombinant thrombin to bovine thrombin as a hemostatic ancillary in patients undergoing peripheral arterial bypass and arteriovenous graft procedures. PMID:18440754
Thrombomoduln not only binds to HMGB1 but also aids the proteolytic cleavage of HMGB1 by thrombin. PMID:18599803
Examination of a dipalmitoylphosphatidylcholine membrane-bound prothrombin 1 fragment reveals another well-defined carboxyglutamic acid (Gla domain)-binding site for phosphatidylserine. PMID:19086158
These studies illustrate that GpIbalpha and gamma' target thrombin binding sites with similar consequences on thrombin dynamics, albeit with differing structural features. PMID:19591434
ERK-dependent IKKalpha/beta/NF-kappaB signaling pathway plays an important role in thrombin-induced COX-2 expression in human lung fibroblasts. PMID:19616539