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Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)

Recombinant Bovine Palmitoyl-protein thioesterase 1 (PPT1)

Baculovirus
Mammalian cell

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PPT1

Uniprot NO.

P45478

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

28-306

Target Protein Sequence

DPP APLPLVIWHG MGDSCCNPLS MGAIKKMVEK KIPGIHVLSL EIGKTLREDV ENSFFLNVNS QVTTVCQILA KDPKLQQGYN AMGFSQGGQF LRAVAQRCPS PPMVNLISVG GQHQGVFGLP RCPGESSHIC DFIRKTLNAG AYNKAIQERL VQAEYWHDPI REDIYRNHSI FLADINQERG VNESYKKNLM ALKKFVMVKF LNDTIVDPVD SEWFGFYRSG QAKETIPLQE STLYTQDRLG LKAMDKAGQL VFLALEGDHL QLSEEWFYAH IIPFLE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

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■ Q&A

Regarding your protein CSB-EP018587BO, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information?
Also, have any customers reported this protein to be active?

Thanks for your inquiry.
Recombinant Bovine Palmitoyl-protein thioesterase 1(PPT1)
CSB-EP018587BO >> E.coli
Expression Region: 28-306aa; Full Length of Mature Protein
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

Sorry, as this is a semi-custom protein, which means we have never expressed any of these proteins before, so also haven't tested the activity or received the feedback from other customers, we can't 100% guarantee its activity.However, as what we provide is the full length of the mature protein, and it's purified under very mild condition, we recommend you to purchase a small size of eukaryotic expressed protein to have a try.
Generally, the more advanced expression system is the protein expressed with, the more likely to be active.
Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839016/
This literature introduces that the Human protein expressed by the Mammalian cell expression system is ative, while the homology between bovine and human protein is 94%. So in theory, the bovine protein should also be active.

Target Background

Function(From Uniprot)

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

Subcellular Location

Lysosome. Secreted.

Protein Families

Palmitoyl-protein thioesterase family

Tissue Specificity

Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle.

Database Links

UNIGENE: Bt.5019

KEGG: bta:281421

STRING: 9913.ENSBTAP00000017780

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