Recombinant Bovine Osteopontin (SPP1)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SPP1

Uniprot NO.

P31096

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

17-278

Target Protein Sequence

LPVK PTSSGSSEEK QLNNKYPDAV ATWLKPDPSQ KQTFLAPQNS VSSEETDDNK QNTLPSKSNE SPEQTDDLDD DDDNSQDVNS NDSDDAETTD DPDHSDESHH SDESDEVDFP TDIPTIAVFT PFIPTESAND GRGDSVAYGL KSRSKKFRRS NVQSPDATEE DFTSHIESEE MHDAPKKTSQ LTDHSKETNS SELSKELTPK AKDKNKHSNL IESQENSKLS QEFHSLEDKL DLDHKSEEDK HLKIRISHEL DSASSEVN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Major non-collagenous bone protein that binds tightly to hydroxyapatite (Probable). Appears to form an integral part of the mineralized matrix (Probable). Probably important to cell-matrix interaction (Probable).; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Gene References into Functions

study first addressed in detail the LF/fOPN interaction and proposed a LF:fOPN 4/1 maximal stoichiometry. PMID:28561851
This implies that a majority of the acidic residues within OPN must be engaged in calcium interaction under physiological conditions. PMID:24928493
Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk. PMID:24961487
Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells. PMID:25316140
osteopontin (OPN) can serve as a process-directing agent for the intrafibrillar mineralization of collagen. PMID:24140612
Results suggest a mechanism of the interaction of UO2(2+) with bone metabolism and a new role for osteopontin (OPN) as a metal transporter. PMID:24327307
Upon induction of luteolysis, SPP1 serves as a signaling molecule to recruit or activate immune cells to facilitate luteal regression and tissue degradation. PMID:24019509
OPN strongly reduces the amount of biofilm formed in a well-defined laboratory model of acidogenic dental biofilm. PMID:22879891
This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine milk osteopontin (bmOPN) with post-translational modifications (PTMs) compared to human Escherichia-coli-derived recombinant OPN without PTMs. PMID:19854170
topographical distribution of both the in vivo and in vitro phosphorylation sites of bone sialoprotein PMID:15004024
the bovine osteopontin gene has two functionally distinct clusters of haplotypes within the QTL region on chromosome 6 PMID:15867146
The relationship between polymorphisms in SPP1 and several factors affecting growth and development in beef cattle was studied. PMID:17179534
findings show significant association of osteoponitin variants with milk composition traits PMID:17517737
In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII. PMID:17615552
The binding of bovine seminal proteins A1, A2, and 30 kDa and osteopontin to spermatozoa and the effects of oviductal fluid on protein binding are reported. PMID:18207674
Results of this study confirm for the first time, the expression of Opn in the ileum and ileocecal lymph node of cattle with paratuberculosis. PMID:18620757
Opn may be a key regulator against Mycobacterium avium ssp. paratuberculosis infection PMID:18650284
The role of OPN phosphate groups in adsorption to, incorporation into and inhibition of calcium oxalate monohydrate crystals was studied by comparing OPN isoforms differing in phosphorylation. PMID:18703867
SPP1 (also known as osteopontin) has a significant role in the modulation of milk protein gene expression. PMID:19392827
OPN may act as a carrier protein for LF in milk, and modulate the potent antimicrobial and immunostimulatory activities of the LF protein. PMID:19477017
the present study is the first to demonstrate functionally that SPP1 directly binds specific integrins to promote trophectoderm cell migration and attachment to luminal epithelium that may be critical to conceptus elongation and implantation. PMID:19571258
Polymorphisms in the genomic sequence of SPP1 can influence the number of somatic cells in milk and, potentially, influence susceptibility to mastitis. PMID:19765294