Home
Product
Protein
Recombinant Proteins
Recombinant Bovine Nitric oxide synthase, inducible (NOS2), partial

Recombinant Bovine Nitric oxide synthase, inducible (NOS2), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Abbreviation

NOS2

Purity

>85% (SDS-PAGE)

Target Names

NOS2

Uniprot NO.

Q27995

Alternative Names

NOS2; Nitric oxide synthase; inducible; EC 1.14.13.39; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

1-1156

Target Protein Sequence

MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG KHGNESPQPL TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL HQKAKGDLSC KSKSCLASIM NPKSLTIGPR DKPTPPDELL PQAIEFVNQY YGSFKEAKIE EHLARVEAVT KEIETTGTYQ LTGDELIFAT KQAWRNAPRC IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI RSAITVFPQR SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP AVANMLLEVG GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG LETHKVASLW KDRAVVEINV AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE YRSRGGCPAD WIWLVPPISG SITPVFHQEM LNYVLSPFYY YQVEPWKTHV WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT ILFATETGRS ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA SQLAPTGEGD ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS NVTWDPQHYR LVQDSEPLDL NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ PALVQGILER VVDGPAPHQP VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ LLLRKLAQLA TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS LKPQDPVPCF VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA EHKGLQGGRM TLVFGCRRPE EDHLYWEEML EMARKGVLHE VHTAYSRLPD QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY VCGDVRMARD VARTLKQLMA TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG AAGLPSNPRA PGAHRS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.

Gene References into Functions

polymorphism of the NOS2 gene may contribute to the susceptibility of Holstein cattle to bovine tuberculosis PMID:26468216
Vitamin D receptor activation, and inducible nitric oxide synthase (NOS2), were strongly induced during Cooperia oncophora reinfection. Several canonical pathways associated with NOS2 were impacted. PMID:21414188
The expression, localization, and distribution of 3 nitric oxide synthase enzymes through the estrous cycle in bovien fallopian tubes are reported. PMID:23410122
These results provide evidence for a high prevalence of subclinical endometritis in repeat breeding cows as well as the involvement of TNFalpha and iNOS pathways in the regulation of this pathological condition. PMID:24597304
Differential cell-specific and spatiotemporal expression of the EDN1 system and NOS in the bovine utero-placental unit may be associated with regulation of vascular and cellular functions during pregnancy. PMID:22921266
All infected calves had an increased number of cells expressing iNOS, nitrotyrosine and manganese superoxide dismutase in the inflamed lung tissue PMID:21334636
iNOS is expressed in ruminant granulosa cells and is regulated by gonadotrophins and oestradiol PMID:21256181
Expression level of NOS2 mRNA in endometrial biopsies from cows with puerperal endometritis is high. Highest expression of is found in cows with clinical endometritis. PMID:20414720
data suggest that the transcriptional inducible NOS response to octacalcium phosphate crystals involved both the p38 and the JNK MAPK pathways, probably under the control of activator protein-1 PMID:16207333
The expression of iNOS and NRAMP1 in the lymph nodes, lungs, and tuberculous granulomas in 8 bovine tuberculosis cases is reported. PMID:16966449
identified a 3471bp transcript for iNOS, isolated from RNA from alveolar macrophages stimulated with the intracellular pathogen Mycobacterium bovis PMID:17336395
The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected. PMID:17530193
fibronectin fragment induced iNOS and COX-2 expression is downregulated in chondrocyte/agarose constructs PMID:18677626
study investigated hypothesis that CD40 signalling is impaired in Mycobacterium avium subspecies paratuberculosis(MAP)-infected macrophages; propose that MAP interferes with gene expression of iNOS and IL-12p40 genes downstream of p38 PMID:19022505
Isoforms of NOS (eNOS and iNOS) are present in oocytes of cattle from early folliculogenesis up to maturation; in vitro maturation influences amount of mRNA and NOS activity. PMID:19327921