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Recombinant Bovine Neutrophil cytosol factor 1 (NCF1)

Recombinant Bovine Neutrophil cytosol factor 1 (NCF1)

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

NCF1

Uniprot NO.

O77774

Alternative Names

NCF1; Neutrophil cytosol factor 1; NCF-1; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; p47-phox

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

1-392

Target Protein Sequence

MGDHFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKILKEMFPI EAGDINPENR IIPHLPAPRW YDGQRVAESR QGTLTEYCST LMSLPVKISR CPHLLNFFKV RPDDLKLPTD SQVKKPETYL MPKDGKNNAA DITGPIILQT YRAIADYEKG SSSQMALATG DVVDVVEKNE SGWWFCQMKT KRGWVPASYL EPLDSPDEAE DPEPNYAGEP YITIKAYTAV LEDEISLEEG EAIEVIHKLL DGWWVIRKED VTGYFPSMYL QKAGQDVAQA KSQIKSRGAP PRRSSIRNAH SIHQRSRKRL SQDTYRRNSV RFMQQRRHQR PEPQRSRSAL REQQQPKTER PKPQPAVPPR PSADLILHRC SESTKRKLAS AV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Gene References into Functions

Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress. PMID:26826128
two novel splice variants designed as NCF1-TV1 (retained intron 6) and NCF1-TV2 (retained part of intron 8)encoding two putative truncated proteins (239AA and 283AA). Two splice variants were up-regulated in the mastitis-infected cows' mammary tissues, blood and neutrophils compared with these of healthy cows. PMID:25946326
A p47(phox) and Src kinase activation of peroxide production by Nox2 appears to be an important contributor to vascular contractile mechanisms mediated through activation of protein kinase C. PMID:19168729

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Subcellular Location

Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side.

Database Links

UNIGENE: Bt.142

KEGG: bta:281345

STRING: 9913.ENSBTAP00000004279

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