Recombinant Bovine Homeobox protein NANOG (NANOG)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Target Background

Product Details

Abbreviation

NANOG

Purity

>85% (SDS-PAGE)

Target Names

NANOG

Uniprot NO.

Q4JM65

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

1-300

Target Protein Sequence

MSVGPACPQS LLGPEASNSR ESSPMPEESY VSLQTSSADT LDTDTVSPLP SSMDLLIQDS PDSSTSPRVK PLSPSVEEST EKEETVPVKK QKIRTVFSQT QLCVLNDRFQ RQKYLSLQQM QELSNILNLS YKQVKTWFQN QRMKCKKWQK NNWPRNSNGM PQGPAMAEYP GFYSYHQGCL VNSPGNLPMW GNQTWNNPTW SNQSWNSQSW SNHSWNSQAW CPQAWNNQPW NNQFNNYMEE FLQPGIQLQQ NSPVCDLEAT LGTAGENYNV IQQTVKYFNS QQQITDLFPN YPLNIQPEDL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator and repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Gene References into Functions

NANOG was absent or very faint in OCT4 knockout blastocysts. PMID:29483258
analysis of pluripotency gene expression of OCT4, SOX2 and NANOG and mRNA levels of some of their downstream targets in bovine oocytes and early embryos PMID:22479535
essential for induction of pluripotency in adult fibroblasts PMID:21478453
Nanog displayed relatively the same methylation levels between sperm and oocytes PMID:20604984
Noggin, a cytokine inhibiting the BMP4 pathway, successfully upregulated the relative expression of NANOG mRNA in the ICM explants with respect to controls. PMID:19594391