Recombinant Bovine Collagenase 3 (MMP13)

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MMP13

Uniprot NO.

O77656

Species

Bos taurus (Bovine)

Source

Yeast

Expression Region

104-471

Target Protein Sequence

YNVFPRT LKWSKMNLTY RIVNYTPDLT HSEVEKAFRK AFKVWSDVTP LNFTRIHNGT ADIMISFGTK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYTYTGKS HFMLPDDDVQ GIQSLYGPGD EDPYSKHPKT PDKCDPSLSL DAITSLRGET LIFKDRFFWR LHPQQVEAEL FLTKSFGPEL PNRIDAAYEH PSHDLIFIFR GRKFWALSGY DILEDYPKKI SELGFPKHVK KISAALHFED SGKTLFFSEN QVWSYDDTNH VMDKDYPRLI EEVFPGIGDK VDAVYQKNGY IYFFNGPIQF EYSIWSNRIV RVMTTNSLLW C
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

Gene References into Functions

p38 phosphorylation and MMP13 expression are regulated by Rho/ROCK activation, and support the potential novel pathway that Rho/ROCK is in the upper part of the mechanical stress-induced matrix degeneration cascade in cartilage. PMID:22688963
These data identify atypical PKC isozymes as STAT and ERK activators that mediate c-fos and collagenase expression. PMID:20463008
MMP-13 treatment of fresh articular cartilage results in cleaved fibromodulin fragments PMID:14660626
increased in bovine preovulatory follicles following the gonadotropin surge but no up-regulation of MMP-1 and MMP-13 (follicular apex vs. base) for the preovulatory collagenolysis required for follicle rupture PMID:15115716
involvement of p38 MAP kinase in the hyaluronan oligosaccharide induction of MMP-13 PMID:16648633
MMP-13 and collagenase have roles in chondrocyte hypertrophy induced by type II collagen PMID:17306969
At high but naturally occurring concentrations the collagen peptide CB12-II induced an increase in the expressions of MMP-13 and cleavage of type II collagen by collagenase in the mid zone and also in the superficial zone. PMID:18589731