Very nice to receive your inquiry.
1. Full length protein:
Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B
CSB-CF337356ANE
Expression Region: 26-626aa; Mature full length protein.
Tag information:N-terminal 6xHis-tagged
Sequence:
KSSPYQKKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDPRGHTGTTNQRSPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAGPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSHVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSRQFQNLQNHRIVQIEAKPNTLVLPKHADADNILVIQQGQATVTVANGNNRKSFNLDEGHALRIPSGFISYILNRHDNQNLRVAKISMPVNTPGQFEDFFPASSRDQSSYLQGFSRNTLEAAFNAEFNEIRRVLLEENAGGEQEERGQRRWSTRSSENNEGVIVKVSKEHVEELTKHAKSVSKKGSEEEGDITNPINLREGEPDLSNNFGKLFEVKPDKKNPQLQDLDMMLTCVEIKEGALMLPHFNSKAMVIVVVNKGTGNLELVAVRKEQQQRGRREEEEDEDEEEEGSNREVRRYTARLKEGDVFIMPAAHPVAINASSELHLLGFGINAENNHRIFLAGDKDNVIDQIEKQAKDLAFPGSGEQVEKLIKNQKESHFVSARPQSQSQSPSSPEKESPEKEDQEEENQGGKGPLLSILKAFN
2. The full length protein expressed by other four expression systems with the Cat No CSB-XP337356ANE series are discontinued, as the quality is not very good.Now we can only provide this full length protein expressed by the cell free expression system.
3. The partial protein expressed by the other four expression systems with Cat No CSB-XP337356ANE1 series are available.
Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B,partial
CSB-YP337356ANE1 >> Yeast
CSB-EP337356ANE1 >> E.coli
CSB-BP337356ANE1 >> Baculovirus
CSB-MP337356ANE1 >> Mammalian cell
Expression Region: 170-586aa; Partial.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:
SRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSRQFQNLQNHRIVQIEAKPNTLVLPKHADADNILVIQQGQATVTVANGNNRKSFNLDEGHALRIPSGFISYILNRHDNQNLRVAKISMPVNTPGQFEDFFPASSRDQSSYLQGFSRNTLEAAFNAEFNEIRRVLLEENAGGEQEERGQRRWSTRSSENNEGVIVKVSKEHVEELTKHAKSVSKKGSEEEGDITNPINLREGEPDLSNNFGKLFEVKPDKKNPQLQDLDMMLTCVEIKEGALMLPHFNSKAMVIVVVNKGTGNLELVAVRKEQQQRGRREEEEDEDEEEEGSNREVRRYTARLKEGDVFIMPAAHPVAINASSELHLLGFGINAENNHRIFLAGDKDNVIDQIEKQAKDLAFPGSGEQVEKLIKNQKESHFVSAR
The fragment (170-586aa) that we provide is the core region. The crystal structure of the Ara h 1 core (residues 170–586) shows that the central part of the allergen has a bicupin fold,which is in agreement with our bioinformatics analysis. In its crystalline state, the core region of Ara h 1 forms trimeric assemblies, while in solution the protein exists as higher molecular weight assemblies.
This finding reveals that the residues forming the core region of the protein are sufficient for formation of Ara h 1 trimers and higher order oligomers. )
Reference Link:http://europepmc.org/articles/PMC3234756;jsessionid=DF4A0BC947CA94810565990956689634
In addition, both of the CSB-EP331779ANE and CSB-EP337356ANE are the Arachis hypogaea Allergen Ara h 1.
Reference: https://en.wikipedia.org/wiki/Ara_h1
Difference between the follonwing two products:
CSB-EP331779ANE is Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 Reference: http://www.uniprot.org/uniprot/P43237
CSB-EP337356ANE is Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B Reference: http://www.uniprot.org/uniprot/P43238
The epitopes of these two proteins:http://www.iedb.org/epitope_tab.php?srcId=91827&total_rows=4782&column_name=source_organism&sort=desc&page=186
The homology between these two proteins is 95%, Signal peptide sequences are basically the same, amino acid sequence differences are mainly concentrated at the N-terminus and C-terminus.
If you are interested in the last clone of the protein name, we guess it may be named by the subclone name of the human immune cell (ie, the corresponding number on the 96-well plate) when designing and implementing the experimental screen peanut allergen.
CSB-XP331779ANE series:
We have successfully expressed both of this partial protein and full length protein, please check the details as follows:
1. Full length protein:
Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 ,partial
CSB-YP331779ANE
CSB-EP331779ANE
CSB-BP331779ANE
CSB-MP331779ANE
Expression Region: 26-216aa; Partial.
Tag information:EP: N-terminal 6xHis-SUMO-tagged; YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for other expression systems:
YP: N-terminal 6xHis-tagged; BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:
KSPYRKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDTGATNQRHPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAEPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSEVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSKQFQNLQNHRIVQIEARPN
The EP protein is successfully expressed with N-terminal 6xHis-SUMO-tagged.
2. Partial protein:
Recombinant Arachis hypogaea Allergen Ara h 1, clone P17
CSB-YP331779ANE(A4) >> Yeast
CSB-EP331779ANE(A4) >> E.coli
CSB-BP331779ANE(A4) >> Baculovirus
CSB-MP331779ANE(A4) >> Mammalian cell
Expression Region: 26-614aa; Mature full length protein.
Tag information:EP: N-terminal 6xHis-tagged;YP: N-terminal 10xHis-tagged; BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for other expression systems: BP, MP:N-terminal 6xHis-tagged
Sequence:KSPYRKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDTGATNQRHPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAEPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSEVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSKQFQNLQNHRIVQIEARPNTLVLPKHADADNILVIQQGQATVTVANGNNRKSFNLDEGHALRIPSGFISYILNRHDNQNLRVAKISMPVNTPGQFEDFFPASSRDQSSYLQGFSRNTLEAAFNAEFNEIRRVLLEENAGGEQEERGQRRRSTRSSDNEGVIVKVSKEHVQELTKHAKSVSKKGSEEEDITNPINLRDGEPDLSNNFGRLFEVKPDKKNPQLQDLDMMLTCVEIKEGALMLPHFNSKAMVIVVVNKGTGNLELVAVRKEQQQRGRREQEWEEEEEDEEEEGSNREVRRYTARLKEGDVFIMPAAHPVAINASSELHLLGFGINAENNHRIFLAGDKDNVIDQIEKQAKDLAFPGSGEQVEKLIKNQRESHFVSARPQSQSPSSPEKEDQEEENQGGKGPLLSILKAFN
The EP protein is successfully expressed with N-terminal 6xHis-tag.
The YP protein is successfully expressed with N-terminal 10xHis-tag.
