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Recombinant Arabidopsis thaliana Ubiquitin-activating enzyme E1 1 (UBA1), partial

Recombinant Arabidopsis thaliana Ubiquitin-activating enzyme E1 1 (UBA1), partial

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MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

UBA1

Uniprot NO.

P93028

Species

Arabidopsis thaliana (Mouse-ear cress)

Source

Yeast

Expression Region

1-1080

Target Protein Sequence

MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR GFHGDSVVQQ IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI SGMHGLGAEI AKNLILAGVK SVTLHDERVV ELWDLSSNFV FSEDDVGKNR ADASVQKLQD LNNAVVVSSL TKSLNKEDLS GFQVVVFSDI SMERAIEFDD YCHSHQPPIA FVKADVRGLF GSVFCDFGPE FAVLDVDGEE PHTGIIASIS NENQAFISCV DDERLEFEDG DLVVFSEVEG MTELNDGKPR KIKSTRPYSF TLDEDTTNYG TYVKGGIVTQ VKQPKLLNFK PLREALKDPG DFLFSDFSKF DRPPLLHLAF QALDHFKAEA GRFPVAGSEE DAQKLISIAT AINTGQGDLK VENVDQKLLR HFSFGAKAVL NPMAAMFGGI VGQEVVKACS GKFHPLFQFF YFDSVESLPS EPVDSSDFAP RNSRYDAQIS VFGAKFQKKL EDAKVFTVGS GALGCEFLKN LALMGVSCGS QGKLTVTDDD IIEKSNLSRQ FLFRDWNIGQ AKSTVAASAA AVINPRFNIE ALQNRVGAET ENVFDDAFWE NLTVVVNALD NVNARLYVDS RCLYFQKPLL ESGTLGTKCN TQSVIPHLTE NYGASRDPPE KQAPMCTVHS FPHNIDHCLT WARSEFEGLL EKTPAEVNAY LSSPVEYTNS MMSAGDAQAR DTLERIVECL EKEKCETFQD CLTWARLRFE DYFVNRVKQL IYTFPEDAAT STGAPFWSAP KRFPRPLQYS SSDPSLLNFI TATAILRAET FGIPIPEWTK NPKEAAEAVD RVIVPDFEPR QDAKIVTDEK ATTLTTASVD DAAVIDDLIA KIDQCRHNLS PDFRMKPIQF EKDDDTNYHM DVIAGLANMR ARNYSIPEVD KLKAKFIAGR IIPAIATSTA MATGLVCLEL YKVLDGGHKV EAYRNTFANL ALPLFSMAEP LPPKVVKHRD MAWTVWDRWV LKGNPTLREV LQWLEDKGLS AYSISCGSCL LFNSMFTRHK ERMDKKVVDL ARDVAKVELP PYRNHLDVVV ACEDEDDNDV DIPLVSIYFR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Gene References into Functions

One mutant that suppresses snc1-mediated constitutive resistance is modifier of snc1 5 (mos5), which carries a 15-bp deletion in the C-terminus of UBA1. PMID:17217463

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Protein Families

Ubiquitin-activating E1 family

Tissue Specificity

Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls.

Database Links

UNIGENE: At.21347

KEGG: ath:AT2G30110

STRING: 3702.AT2G30110.1

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