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Recombinant Arabidopsis thaliana Phosphoenolpyruvate carboxykinase [ATP] (PCKA), partial

Recombinant Arabidopsis thaliana Phosphoenolpyruvate carboxykinase [ATP] (PCKA), partial

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MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

PCKA

Purity

>85% (SDS-PAGE)

Target Names

PCKA

Uniprot NO.

Q9T074

Alternative Names

PCKA; At4g37870; T28I19.150; Phosphoenolpyruvate carboxykinase; ATP; PEP carboxykinase; PEPCK; EC 4.1.1.49

Species

Arabidopsis thaliana (Mouse-ear cress)

Source

Yeast

Expression Region

1-671

Target Protein Sequence

MSAGNGNATN GDGGFSFPKG PVMPKITTGA AKRGSGVCHD DSGPTVNATT IDELHSLQKK RSAPTTPINQ NAAAAFAAVS EEERQKIQLQ SISASLASLT RESGPKVVRG DPAEKKTDGS TTPAYAHGQH HSIFSPATGA VSDSSLKFTH VLYNLSPAEL YEQAIKYEKG SFITSNGALA TLSGAKTGRA PRDKRVVRDA TTEDELWWGK GSPNIEMDEH TFMVNRERAV DYLNSLEKVF VNDQYLNWDP ENRIKVRIVS ARAYHSLFMH NMCIRPTQEE LESFGTPDFT IYNAGQFPCN RYTHYMTSST SVDLNLARRE MVILGTQYAG EMKKGLFSVM HYLMPKRRIL SLHSGCNMGK DGDVALFFGL SGTGKTTLST DHNRYLIGDD EHCWTETGVS NIEGGCYAKC VDLSREKEPD IWNAIKFGTV LENVVFDEHT REVDYSDKSV TENTRAAYPI EFIPNAKIPC VGPHPTNVIL LACDAFGVLP PVSKLNLAQT MYHFISGYTA LVAGTEDGIK EPTATFSACF GAAFIMLHPT KYAAMLAEKM KSQGATGWLV NTGWSGGSYG VGNRIKLAYT RKIIDAIHSG SLLKANYKKT EIFGFEIPTE IEGIPSEILD PVNSWSDKKA HKDTLVKLGG LFKKNFEVFA NHKIGVDGKL TEEILAAGPI F
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.

Gene References into Functions

Malate metabolism is important during dark-induced stomatal closure and that phosphoenolpyruvate carboxykinase (PEPCK) is involved in this process. PMID:22007864

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Subcellular Location

Cytoplasm.

Protein Families

Phosphoenolpyruvate carboxykinase (ATP) family

Tissue Specificity

Expressed in cotyledons, flowers, siliques, seeds, leaves, stems and roots. Localized in mid-veins.

Database Links

UNIGENE: At.22897

KEGG: ath:AT4G37870

STRING: 3702.AT4G37870.1

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