Recombinant Arabidopsis thaliana Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic (CRD1)

Product Details

Abbreviation
CRD1
Purity
>85% (SDS-PAGE)
Target Names
CRD1
Uniprot NO.
Alternative Names
CRD1; ACSF; AT103; CHL27; ZIP; At3g56940; F24I3.20Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase; chloroplastic; Mg-protoporphyrin IX monomethyl ester oxidative cyclase; EC 1.14.13.81; Copper response defect 1 protein; Dicarboxylate diiron protein; AtZIP; MPE-cyclase
Species
Arabidopsis thaliana (Mouse-ear cress)
Source
Yeast
Expression Region
37-409
Target Protein Sequence
MSAS SSPPPPTTAT SKSKKGTKKE IQESLLTPRF YTTDFEEMEQ LFNTEINKNL NEAEFEALLQ EFKTDYNQTH FVRNKEFKEA ADKLQGPLRQ IFVEFLERSC TAEFSGFLLY KELGRRLKKT NPVVAEIFSL MSRDEARHAG FLNKGLSDFN LALDLGFLTK ARKYTFFKPK FIFYATYLSE KIGYWRYITI YRHLKENPEF QCYPIFKYFE NWCQDENRHG DFFSALMKAQ PQFLNDWQAK LWSRFFCLSV YVTMYLNDCQ RTNFYEGIGL NTKEFDMHVI IETNRTTARI FPAVLDVENP EFKRKLDRMV VSYEKLLAIG ETDDASFIKT LKRIPLVTSL ASEILAAYLM PPVESGSVDF AEFEPNLVY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)
Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME).
Gene References into Functions
  1. chl27-t knock-down mutants exhibit retarded growth and chloroplast developmental defects that are caused by damage to PSII reaction centers. Mutants also have significant repression of genes for chlorophyll biosynthesis and iron acquisition. PMID:18682427

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Subcellular Location
Plastid, chloroplast inner membrane; Peripheral membrane protein. Plastid, chloroplast thylakoid membrane; Peripheral membrane protein.
Protein Families
AcsF family
Database Links

UNIGENE: At.48778

KEGG: ath:AT3G56940

STRING: 3702.AT3G56940.1

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