Recombinant Adenylate cyclase (cya)

Baculovirus
Mammalian cell

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

cya

Uniprot NO.

P27580

Species

Glutamicibacter nicotianae (Arthrobacter nicotianae)

Source

Yeast

Expression Region

1-403

Target Protein Sequence

MSTEHTNTPR ADSPQSAAEA VRGARQHAPA ATPAESDPIL ELAEAMEGPL RIPAHTPEAV RDTVASLEKR LIGGQREFRR REVASEAGVS LHSARKLWRA IGFPELSDDE VFFTEADKKA LGTMVGMVRE GALTEETAIS LMRSVGQMTD RMVVWQIEAL VEDMIANQNL SDRQARRQLF SLLPEIIPAI EDLLLYSWRR QLNSAVHRMA LRVETGVAAY NQDRGEDDGG TPLPLARAVG FADLVSYTSL SRRMNERTLA QLVQRFEAKC AEIISVGGGR LVKTIGDEVL YVAETPQAGA QIALSLSREL AKDELFPQTR GAVVWGRLLS RLGDIYGPTV NMAARLTSLA EPGTVLTDAI TANTLRNDAR FVLTAQEITA VRGFGDIQPY ELSAGEGAGL VID
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

full length protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

This recombinant Glutamicibacter nicotianae Adenylate cyclase (cya) is a semi-custom product. There are 5 expression system options: Yeast, E. coli, In Vivo Biotinylation in E. coli, Baculovirus, and Mammalian cell. Your requirements will be given top priority in determining the protein tags. For proteins within 800 aa, risk-free custom service is guaranteed. It means you will not be charged if the protein cannot be delivered.

Adenylate cyclase is an enzyme that hydrolyzes the adenosine triphosphate (ATP) into cyclic adenosine monophosphate (cAMP), a crucial secondary messenger in various cellular signaling pathways [1]. It mediates responses to extracellular signals by regulating cAMP levels, which activates downstream effectors such as protein kinase A (PKA) [2]. Opiates have been found to inhibit this enzyme and alter its activity forms [3]. Furthermore, calmodulin regulates adenylate cyclase, activating basal enzyme activity and potentiating activities involving GTP-binding proteins [4].

References:
[1] C. Catanese, D. Emerich, & W. Zahler, Adenylate cyclase and cyclic amp phosphodiesterase in bradyrhizobium japonicum bacteroids, Journal of Bacteriology, vol. 171, no. 9, p. 4531-4536, 1989. https://doi.org/10.1128/jb.171.9.4531-4536.1989
[2] P. Barrett, C. Schuster, J. Mercer, & P. Morgan, Sensitization: a mechanism for melatonin action in the pars tuberalis, Journal of Neuroendocrinology, vol. 15, no. 4, p. 415-421, 2003. https://doi.org/10.1046/j.1365-2826.2003.00988.x
[3] S. Sharma, W. Klee, & M. Nirenberg, Opiate-dependent modulation of adenylate cyclase., Proceedings of the National Academy of Sciences, vol. 74, no. 8, p. 3365-3369, 1977. https://doi.org/10.1073/pnas.74.8.3365
[4] J. Harrison, C. Mickevicius, & M. Gnegy, Differential regulation by calmodulin of basal, gtp‐, and dopamine‐stimulated adenylate cyclase activities in bovine striatum, Journal of Neurochemistry, vol. 51, no. 2, p. 345-352, 1988. https://doi.org/10.1111/j.1471-4159.1988.tb01045.x