mutL Antibody, Biotin conjugated

Code: CSB-PA543397HD01ENV

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Size:

50μg100μg

US$166

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Species Reactivity: Escherichia coli

Raised in: Rabbit

Application: ELISA

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Target Background

Product Details

Uniprot NO.

P23367

Target Names

mutL

Alternative Names

mutL antibody; b4170 antibody; JW4128 antibody; DNA mismatch repair protein MutL antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli DNA mismatch repair protein MutL protein (1-615AA)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Biotin

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA

Protocols

ELISA Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

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Target Background

Function(From Uniprot)

This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA.

Gene References into Functions

In this review, we describe biochemical, biophysical and structural analyses that have clarified how MutL aids at discriminating the newly synthesized strand from its template and marking it for removal. [review] PMID:25701376
study found the deletion or insertion of a single LA repeat did not compromise the structural integrity of the protein, nor did it affect MutS- or DNA-binding activity;it severely compromised ATP binding and engagement of the N-terminal domains; both essential activities for proper DNA mismatch repair PMID:23916559
MutL accumulates from the mismatch site toward strand discrimination site along the DNA. PMID:22241777
Evidence for ATP-dependent structural rearrangement of nuclease catalytic site in DNA mismatch repair endonuclease MutL PMID:21953455
C-terminal domain of MutL encompasses a bona fide DNA polymerase III beta-binding motif that mediates a weak, yet specific, interaction between the two proteins. PMID:21050827
The presence of phosphate minimizes further MutL oligomerization beyond a dimer PMID:21793594
Studies indicate that a stable MutL-ssDNA interaction is unlikely to occur at physiological salt. PMID:21103398
MutL homologues play a key role in determining biologic outcome by recruiting and/or activating effector proteins in response to lesion recognition by MutS. PMID:19953589
Data report the crystal structure of the Escherichia coli MutL C-terminal dimerization domain and the likelihood of its conservation among MutL homologs. PMID:15470502
revised model for the biological dimer, which has important implications for understanding the heterodimerization of eukaryotic MutL homologues, modeling the MutL holoenzyme and predicting protein-protein interaction sites PMID:16024043
MutL determines effective DNA homology in recombination processes PMID:17502621
An increase in mutL gene copies was also able to partially compensate the hypermutator phenotype of a mutS-defective E. coli derivative. PMID:17825069
Results suggest a mobile MutS-MutL complex in DNA mismatch repair signalling, that leaves the DNA mismatch prior to, or at the time of, activation of downstream effector molecules such as Vsr or MutH. PMID:19474347