ligA Antibody

Code: CSB-PA322745HA01ENV

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US$83

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Species Reactivity: Escherichia coli

Raised in: Rabbit

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P15042

Target Names

ligA

Alternative Names

ligA antibody; dnaL antibody; lig antibody; lop antibody; pdeC antibody; b2411 antibody; JW2403 antibody; DNA ligase antibody; EC 6.5.1.2 antibody; Polydeoxyribonucleotide synthase [NAD(+)] antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli DNA ligase protein (1-671AA)

Immunogen Species

Escherichia coli

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: DNA ligase antibody at 2μg/ml + DH5a whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa

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Target Background

Function(From Uniprot)

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Gene References into Functions

Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID:26857547
Here the authors show that replication without ligase kills after a short resistance period. PMID:22582878
temperature sensitive mutant characterized PMID:15583169
analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID:15671015
The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID:15848142
2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID:17466627
structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID:18515356