Tet Antibody

Code: CSB-PA145385XA11DLU

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Fruit fly

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Uniprot NO.

M9NEY8

Target Names

Tet

Alternative Names

DNA N6-methyl adenine demethylase (EC 1.14.11.51) (DNA 6mA demethylase), Tet, DMAD

Species Reactivity

Fruit fly

Immunogen

Recombinant Fruit fly Tet protein (2494-2767aa)

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: recombinant protein
All lanes: Tet Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa

Description

The Tet polyclonal antibody is generated by introducing the recombinant fruit fly Tet protein (amino acids 2494-2767) into a rabbit, stimulating the production of antibodies targeting this antigen. A booster injection enhances antibody production, and the resulting Tet polyclonal antibodies in the rabbit's serum are collected and purified using affinity chromatography. Extensive testing in ELISA and WB assays has confirmed the Tet antibody's specific recognition of fruit fly Tet protein.

The fruit fly genome encodes a family of Tet proteins, including Tet1, Tet2, and Tet3, which play a crucial role in the dynamic regulation of DNA methylation and gene expression. These proteins are responsible for the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) during DNA demethylation, which contributes to various developmental and cellular processes in fruit flies.

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Target Background

Function(From Uniprot)

Dioxygenase that specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA. N(6)-methyladenosine (m6A) DNA is present at a relatively high level at the very earliest embryonic stages but at low levels at the late embryonic stages and may act as a regulator of gene expression. Promotes differentiation of early germ cells in ovary.

Protein Families

TET family

Database Links

UNIGENE: Dm.2189

KEGG: dme:Dmel_CG43444

STRING: 7227.FBpp0306013

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