SAGE1 Antibody

Code: CSB-PA020670LA01HU

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

Q9NXZ1

Target Names

SAGE1

Alternative Names

Cancer/testis antigen 14 antibody; CT14 antibody; RP11-97N5.1 antibody; SAGE antibody; SAGE1 antibody; SAGE1_HUMAN antibody; Sarcoma antigen 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Sarcoma antigen 1protein (3-133AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-PA020670LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA020670LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Target Background

Gene References into Functions

ADAM2, CALR3 and SAGE1 cancer/testis antigens are not promising targets for immunotherapy of breast and lung cancer. PMID:26252478
MAGE-A4(143-151) and SAGE(715-723) are HLA-A2402-restricted CTL epitopes PMID:16061876

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Tissue Specificity

Expressed mainly in bladder, lung, head and neck carcinomas. Not expressed in normal tissues except for testis.

Database Links

HGNC: 30369

UNIGENE: Hs.195292

KEGG: hsa:55511

STRING: 9606.ENSP00000323191

OMIM: 300359

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