ROBO2 Antibody

Code: CSB-PA888028ESR1HU

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Size:

20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

Q9HCK4

Target Names

ROBO2

Alternative Names

lea antibody; Robo 2 antibody; Robo2 antibody; ROBO2_HUMAN antibody; Roundabout 2 antibody; Roundabout homolog 2 antibody; roundabout, axon guidance receptor, homolog 2 (Drosophila) antibody; Roundabout2 antibody; SAX 3 antibody; SAX3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Roundabout homolog 2 protein (450-660AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Ntera-2 whole cell lysate
All lanes: ROBO2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 131, 152, 154 kDa
Observed band size: 152 kDa

Description

The production of anti-ROBO2 antibody involves immunizing rabbits with recombinant human ROBO2 protein (450-660aa), collecting the resulting blood to isolate the anti-serum, and purifying the antibody through antigen affinity purification.

The resulting ROBO2 polyclonal antibody is a type of unconjugated IgG. It only recognizes the human ROBO2 protein, which plays a crucial role in axon guidance, cell migration, angiogenesis, and tumor progression. This antibody has undergone validation for use in both ELISA and WB applications, allowing for the detection of the quantity and presence of the ROBO2 protein.

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Target Background

Function(From Uniprot)

Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.

Gene References into Functions

determined the crystal structure of the tandem Ig4-5 domains (D4-5) of human Robo2 and found that a hydrophobic surface on D4 mediates close homotypic contacts with a reciprocal D4 PMID:29017837
ROBO2 variants were not found to be associated with nonsyndromic vesicoureteral reflux with or without renal hypoplasia/dysplasia, providing further evidence for genetic heterogeneity PMID:27002985
ROBO2 and RASSF2A gene methylations may be related to gastric tumorigenesis, and ROBO2 gene methylation is associated with sporadic gastric cancer. PMID:27461616
Overexpression of ROBO2 induces its tumor suppressive effects in Breast cancer PMID:26542734
ROBO2 somatic mutation is associated with myelodysplastic syndrome progression. Overexpression of ROBO2 produces anti-proliferative and pro-apoptotic effects in leukemia cells. This effect was lost in ROBO mutants and ROBO-SLIT2 signaling is impaired. PMID:26608094
Common variation near ROBO2 is associated with expressive vocabulary in infancy. PMID:25226531
Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and SRGAP1 confer risk for congenital anomalies of the kidney and urinary tract PMID:26026792
Down-regulation of ROBO2 expression is associated with prostate cancers. PMID:24272677
The finding that a polymorphism associated with the ROBO2 gene, which is involved in neurodevelopment, confers risk for the common emotionally reactive, impulsive aspects of conduct disorder, independent of concurrent risk for psychopathy. PMID:23982283
Results not completely rule out that some ROBO2 variants may be the sole cause of vesicoureteric reflux, but it is clear from the overall frequency that most of them cannot be. PMID:23536131
two differentially expressed isoforms were isolated and identified. Exons 1 and 2 of human ROBO2a lie in an inherently unstable DNA segment at human chromosome 3p12.3. PMID:16829019
Disruption of ROBO2 is associated with urinary tract anomalies and confers risk of vesicoureteral reflux. PMID:17357069
a relatively high frequency of ROBO2 variants (5.1%) was found in familial vesicoureteral reflux PMID:18235093
mRNA expression of ROBO2 in lymphocytes was significantly reduced in the autistic group PMID:18270976
gene variants in ROBO2 are rare causes of VUR in humans. Our results provide further evidence for the genetic heterogeneity of this disorder PMID:19350278